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- 详细信息
- 技术资料
- 免疫原:
Recombinant human TPPP protein
- 亚型:
IgG2b
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat, pig, rabbit, chicken
- 宿主:
Mouse
- 应用范围:
WB, IHC, IF/ICC, ELISA
- 靶点:
TPPP
- 抗体英文名:
TPPP Monoclonal antibody
- 抗体名:
TPPP Monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | pig brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, SH-SY5Y cells, rabbit brain, rat brain, mouse brain, chicken brain |
| Positive IHC detected in | human lung tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68048-1-Ig targets TPPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 免疫原 |
CatNo: Ag19046 Product name: Recombinant human TPPP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-95 aa of BC131506 Sequence: MADKAKPAKAANRTPPKSPGDPSKDRAAKRLSLESEGAGEGAAASPELSALEEAFRRFAVHGDARATGREMHGKNWSKLCKDCQVIDGRNVTVTD |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tubulin polymerization promoting protein |
| 别名 | p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein |
| 计算分子量 | 219 aa, 24 kDa |
| 观测分子量 | 24-29 kDa |
| GenBank蛋白编号 | BC131506 |
| 基因名称 | TPPP |
| Gene ID (NCBI) | 11076 |
| RRID | AB_2918790 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O94811 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.
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