- ￥1350 - 3000
- Proteintech
- 11221-1-AP
- 2025年10月09日
- WB, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CXCL3 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
CXCL3/GRO Gamma
- 抗体英文名：
CXCL3/GRO Gamma Polyclonal antibody
- 抗体名：
CXCL3/GRO Gamma Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | LPS and Protein transport inhibitor treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11221-1-AP targets CXCL3/GRO Gamma in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1732 Product name: Recombinant human CXCL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-107 aa of BC016308 Sequence: MAHATLSAAPSNPRLLRVALLLLLLVAASRRAAGASVVTELRCQCLQTLQGIHLKNIQSVNVRSPGPHCAQTEVIATLKNGKKACLNPASPMVQKIIEKILNKGSTN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) ligand 3 |
| 别名 | C X C motif chemokine 3, CINC 2b, CXCL/GRO Gamma, CXCL3, GRO gamma, GRO gamma(1 73), GRO3, GROg, Growth regulated protein gamma, MIP 2b, MIP2 beta, MIP2B, SCYB3 |
| 计算分子量 | 11 kDa |
| 观测分子量 | 8-10 kDa |
| GenBank蛋白编号 | BC016308 |
| 基因名称 | CXCL3 |
| Gene ID (NCBI) | 2921 |
| RRID | AB_3669124 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19876 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CXC chemokine ligand 3 (CXCL3) is a member of CXC-type chemokine family that is identified as a major regulator in immune and inflammation responses. CXCL3 is broadly expressed in various human tumor types, and it is also known to play a critical role in mediating tumor development and progression.
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文献和实验| Species | Application | Title |
|---|---|---|
| Human | IF | Nat Commun Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.Authors - Zhaohui Chen |
| human | WB,IF | Cell Death Dis Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinomaAuthors - Tianyin Ma |
实验原理 免疫电泳又称为γ球蛋白电泳或免疫球蛋白电泳技术，这是一种能够判断血液中三种免疫球蛋白IgM，IgG，IgA含量水平的实验方法。在这项实验技术中，首先利用蛋白质的分子量和所带电荷的比值运用水平琼脂糖凝胶电泳技术将蛋白质进行分离，然后将特异性的抗体引入到与电泳方向平行的凹槽中，在抗原和抗体的扩散过程中，抗原和抗体适当比例的结合会导致沉淀的产生。0.85%盐不仅可以终止扩散过程也可用于洗去未结合的蛋白，抗原和抗体结合所形成
相关专题 【实验原理】 免疫电泳又称为γ球蛋白电泳或免疫球蛋白电泳技术，这是一种能够判断血液中三种免疫球蛋白IgM, IgG, IgA含量水平的实验方法。在这项实验技术中，首先利用蛋白质的分子量和所带电荷的比值运用水平琼脂糖凝胶电泳技术将蛋白质进行分离，然后将特异性的抗体引入到与电泳方向平行的凹槽中，在抗原 和抗体的扩散过程中，抗原和抗体适当比例的结合会导致沉淀的产生。0.85%盐不仅可以终止扩散过程也可用
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