- ￥1350 - 3000
- Proteintech
- 31516-1-AP
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
Cathepsin D
- 抗体英文名：
Cathepsin D Polyclonal antibody
- 抗体名：
Cathepsin D Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, HuH-7 cells, MCF-7 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31516-1-AP targets Cathepsin D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cathepsin D |
| 别名 | CTSD, EC:3.4.23.5, CPSD, CLN10, Cathepsin D light chain |
| 计算分子量 | 45kDa |
| 观测分子量 | 32 kDa, 48 kDa, 52 kDa |
| GenBank蛋白编号 | NM_001909.5 |
| 基因名称 | Cathepsin D |
| Gene ID (NCBI) | 1509 |
| RRID | AB_3670015 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P07339 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTSD (cathepsin D) is a lysosomal aspartic protease in the pepsin superfamily ubiquitous to all cell types but particularly abundant in the central nervous system (CNS). The signal peptide of the pre-proCTSD (52 kDa) is cleaved in the endoplasmic reticulum (ER), forming the pro-CTSD (48 kDa), finally processed to form the mature CTSD (32 kDa) in the lysosome (PMID: 31282275,15126630). The major role of the m-CTSD is the digestion of internalized waste cell proteins and peptides in the lysosome, which maintains cell health (PMID: 32324083). CTSD is a well-established aspartyl protease that functions intracellularly in lysosomes and extracellularly in several forms of cancer.
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文献和实验Enzyme Analysis: Cathepsin D as an Example
In enzyme analysis, capillary electrophoresis (CE) offers the ease of product separation from the substrate, with the ability to use expensive reagents in microvolumes. In CE, enzymes can be measured either as mass (when they are present
分为磷酸化一抗、非磷酸化一抗两大类。每一大类又包含若干，不知如何选择，它们之间有何区别？实验目的为研究某细胞AP-1的表达情况，及其蛋白磷酸化和DNA结合活性等。 表达 和 结合 ２个方面都考察? 目的蛋白是ＡＰ-１中的c-jun一个? 不考察c-fos之类的? 还有, 结合可是无法用ＷＢ考察的:D:D chuckdouble ziyunfei wrote:
组织蛋白酶 cathepsin 在各种动物组织的细胞内（特别是溶酶体部分）发现的一类蛋白酶。此名源自希腊语的“消化”，为威尔斯达特（ R． Willstā atter， 1929）命名的。来源于同一种类组织细胞的也是由组织蛋白酶 A． B． C． D． E等多种酶组成。可以根据所作用的代表性底物的种类进行分类，但似乎各自还有数种同工酶。组织蛋白酶 A的代表性底物是 N-苄氧基羰基 -L-谷酰胺 -L-苯丙氨酸，最适 pH约 5.5（ 3— 6），可能是一种羧肽酶，可为二异丙基磷氟酸
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