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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human SECTM1 protein
- 亚型:
IgM
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
SECTM1
- 抗体英文名:
CoraLite® Plus 750-conjugated SECTM1 Monoclonal antibody
- 抗体名:
CoraLite® Plus 750-conjugated SECTM1 Monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive WB detected in | K-562 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-60281 targets SECTM1 in WB applications and shows reactivity with human samples.
| 经测试应用 | WB |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag14645 Product name: Recombinant human SECTM1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 29-248 aa of BC017716 Sequence: QNEGWDSPICTEGVVSVSWGENTVMSCNISNAFSHVNIKLRAHGQESAIFNEVAPGYFSRDGWQLQVQGGVAQLVIKGARDSHAGLYMWHLVGHQRNNRQVTLEVSGAEPQSAPDTGFWPVPAVVTAVFILLVALVMFAWYRCRCSQQRREKKFFLLEPQMKFAALRAGAQQGLSRASAELWTPDSEPTPRPLALVFKPSPLGALELLSPQPLFPYAADP |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | secreted and transmembrane 1 |
| 别名 | K12, Protein K 12, Protein K-12, secreted and transmembrane 1 |
| 计算分子量 | 248 aa, 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC017716 |
| 基因名称 | SECTM1 |
| Gene ID (NCBI) | 6398 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | Q8WVN6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
dH2 O) Denaturing solution (0.5 N NaOH, 1.5 M NaCl) (dissolve 43.83 g NaCl in 300 ml dH2 O, add 50 ml 10 N NaOH stock solution , dH2 O ad 500 ml) Neutralizing solution (1.5 M NaCl, 0.5 M Tris ・ Cl [pH 7.4]) (100 ml dH
抗体所连接的DNA分子,再用电泳来表明实验结果。免疫-PCR的关键之处就在于用一个连接分子将一段特定的DNA连接到抗体上,在抗原和DNA之间建立相对应关系,从而将对蛋白质的检测转变为对核酸的检测。最初Sano等人建立的免疫-PCR实验流程如下:(1)再包被缓冲液稀释抗原BSSA,并固定在微滴定板上。(2)微滴定板上加入相应的已稀释的单克隆抗体,并洗去未结合的抗体分子。(3)加入稀释的已与生物素化PUC19的结合的链亲和素-蛋白A嵌合体(蛋白A能与抗体结合,而链亲和素可与生物素化PUC19中的生物
,用含有10%热灭活胎牛血清的RPMI1640培养基于5% CO2,37C条件下培养。抗CD20单克隆抗体HI47由我所免疫室提供。辣根过氧化酶标记羊抗人IgG,FITC标记的羊抗鼠IgG购于北京中山生物工程公司。2、抗CD20单克隆抗体轻、重链基因的克隆利用RT-PCR法,采用抗体通用的兼并性引物,从HI47杂交瘤细胞特异性地分别扩增抗体轻链、重链可变区基因(VL和VH)后,利用编码短肽(G4S)3的连接链DNA片段,经overlap PCR,将VH和VL基因连接构成ScFv基因(约750bp
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