- ￥5800
- Proteintech
- 83008-5-PBS
- 2025年10月09日
- Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
IL-12/IL-23 p40
- 抗体英文名：
IL-12/IL-23 p40 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
IL-12/IL-23 p40 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
83008-5-PBS targets IL-12/IL-23 p40 as part of a matched antibody pair:
MP00035-4: 83008-5-PBS capture and 83008-6-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg0431 Product name: Recombinant Human IL-12/IL-23 p40 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 23-328 aa of BC067498 Sequence: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) |
| 别名 | IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40, IL 12 subunit p40 |
| 计算分子量 | 328 aa, 37 kDa |
| GenBank蛋白编号 | BC067498 |
| 基因名称 | IL12B |
| Gene ID (NCBI) | 3593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P29460 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
88-7859 TRFK5 JES1-5A10 IL-6 88-7869 MQ2-13A5 MQ2-39C3 IL-10 88-7805 JES3-9D7 JES3-12G8 IL-12 p70 88
Intracellular Immunofluorescent Staining for Flow Cytometry
ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized) + anti-CD28 (2ug/ml soluble) 5hr Brefeldin A JES5-16E3 , JES5-2A5 IL-12/IL-23 (p40)
是没有问题的. 2、表达重组蛋白时，细菌裂解方法都有哪些？ 在表达重组蛋白时，诱导以后跑SDS-PAGE发现表达都很好，但是在裂解细胞时遇到问题。总是不能彻底裂解细胞。 首先我采用超声裂解，可是不管我如何超声总有部分细菌没有裂解。 我的超声条件如下：宁波新芝超声细胞破碎仪，功率400W，超5秒，间隔5秒，重复99次。然后显微镜观察，不彻底时再重复99次。菌体来自200 ml培养液，用20 ml PBS重悬。 然后我又尝试加溶菌酶，首先加至终浓度100 ug/ml，4C半小时菌液不变粘
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