- ￥1350 - 3000
- Proteintech
- 68091-1-Ig
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human BNIP3 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
BNIP3
- 抗体英文名：
BNIP3 Monoclonal antibody
- 抗体名：
BNIP3 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells |
| Positive IHC detected in | mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Cobalt Chloride treated HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68091-1-Ig targets BNIP3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag27935 Product name: Recombinant human BNIP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-194 aa of BC009342 Sequence: MQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDDIERRKEVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSAEFLKVFLPSLLLSHLLAIGLGIYIGRRLTTSTSTF |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | BCL2/adenovirus E1B 19kDa interacting protein 3 |
| 别名 | 2H7D8, NIP3 |
| 计算分子量 | 194 aa, 22 kDa |
| 观测分子量 | 25-30,50-60 kDa |
| GenBank蛋白编号 | BC009342 |
| 基因名称 | BNIP3 |
| Gene ID (NCBI) | 664 |
| RRID | AB_2918828 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q12983 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cellsAuthors - Huafeng Zhang |
| mouse | WB,IHC,IF | Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating MitophagyAuthors - Dan Li |
| mouse | WB | Cell Biosci Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagyAuthors - Mei-Fei Wu |
| human | WB | Cell Rep Proline restores mitochondrial function and reverses aging hallmarks in senescent cellsAuthors - Debanik Choudhury |
| human | WB | Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcomaAuthors - Wenshuo Zhang |
| mouse | WB,IHC | Cell Commun Signal Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1Authors - Xudong Xie |
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