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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
PerCP-Cyanine5.5 Fluorescent Dye
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD45
- 抗体英文名:
PerCP-Cyanine5.5 Anti-Human CD45 (HI30)
- 抗体名:
PerCP-Cyanine5.5 Anti-Human CD45 (HI30)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥2960.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CPY5-65109 targets CD45 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | EC:3.1.3.48, GP180, HI30, L CA, LCA |
| GenBank蛋白编号 | BC014239 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_3673820 |
| 偶联类型 | PerCP-Cyanine5.5 Fluorescent Dye |
| 最大激发/发射波长 | 482 nm / 690 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08575 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
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文献和实验: 由此步骤开始,流出的溶液包含目标细胞,请使用新的试剂管收集!首先,加入 1 ml 的 biotin 洗脱液,孵育 2 min。其次,加入 10 ml biotin 洗脱液,洗脱目标细胞。d) 再加入 10 ml biotin 洗脱液,洗下剩余的目标细胞。最后两步骤所收集的液体含分选出的目标细胞。(4) 结果分析以下流式图为从 7.5 ml 全血中,选出 CD3+ T 细胞的数据。选后细胞以 CD3-APC(OKT-3) 及 CD45-PE(HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞
- 3) 及 CD45 -PE (HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞以 DAPI 染色先行排除,左右分别为分选前后的细胞群分布。 总结: 几次实验的结果,使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选,平均得率为 84.5%,纯度大于 90%,细胞存活率高于 95%,且目标细胞不含标记,贴近天然,使下游实验更精确。
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
antibodies: FITC‐labeled anti‐human CD45 antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555482)
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