- ￥3600 - 5000
- Proteintech
- 98068-1-PBS
- 2025年10月09日
- IHC, FC
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
IHC, FC
- 靶点：
PD-1/CD279
- 抗体英文名：
Anti-Human PD-1/CD279 Rabbit Recombinant Antibody, PBS Only
- 抗体名：
Anti-Human PD-1/CD279 Rabbit Recombinant Antibody, PBS Only
- 规格：
500ug/1mg
| 规格： | 500ug | 产品价格： | ￥3600.0 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ￥5000.0 |
产品信息
98068-1-PBS targets PD-1/CD279 in IHC, FC applications and shows reactivity with human samples.
| 经测试应用 | IHC, FC |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg0974 Product name: Recombinant Human PD-1/CD279 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 24-167 aa of BC074740 Sequence: FLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | programmed cell death 1 |
| 别名 | CD279, PD1, PD-1, 240724G11, hPD 1 |
| 计算分子量 | 288 aa, 32 kDa |
| GenBank蛋白编号 | BC074740 |
| 基因名称 | PD-1 |
| Gene ID (NCBI) | 5133 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q15116 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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