- ￥5800
- Proteintech
- 67155-1-PBS
- 2025年10月09日
- IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SDC1,CD138 protein
- 亚型：
IgG2a
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
CD138/Syndecan-1
- 抗体英文名：
CD138/Syndecan-1 Monoclonal antibody, PBS Only (Detector)
- 抗体名：
CD138/Syndecan-1 Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
67155-1-PBS targets CD138/Syndecan-1 as part of a matched antibody pair:
MP50044-1: 60185-1-PBS capture and 67155-1-PBS detection (validated in Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag0823 Product name: Recombinant human SDC1,CD138 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC008765 Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | syndecan 1 |
| 别名 | CD138, 4F3A8, SDC, SDC1, SYND1 |
| 计算分子量 | 32.5 kDa |
| GenBank蛋白编号 | BC008765 |
| 基因名称 | CD138 |
| Gene ID (NCBI) | 6382 |
| RRID | AB_2882452 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P18827 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.
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文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
过夜。（5）用少许PBS将交联物全部洗出，收集洗出液，加1/20V体积新鲜配制的5mg/ml NaBH4溶液，混匀，室温作用30分钟；再加入3/20V NaBH4溶液，混匀，室温作用1小时（或4℃过夜）。（6）将交联物过Sephadex G200或Sepharose 6B（2.6×50cm）层析纯化，分管收集第一峰。（7）酶结合物质量鉴定：克分子比值测定酶量（mg/ml）=OD403×0.4IgG量（mg/ml）=（OD280-OD403×0.3）×0.62克分子比值（E/P）=酶量×4/IgG量
一、材料及试剂 1、猪瘟单抗纯化抗原 2、兔抗猪IgG酶标抗体 3、猪瘟阳性血清 4、猪瘟阴性血清 1～4均由指定的生物制品所购买。 5、ELISA反应板 6、包被液 碳酸钠 1.50g 碳酸氢钠 2.93g H2O加至 1 000ml pH值9.6 7、PBS液 氯化钠 8.90g 磷酸二氢钾 0.20g 无水磷酸氢二钠 2.13g 加双馏水 1 000ml 8、PBS－吐温洗涤液 PBS液 1 000ml 犊牛血清 5ml 混合即可 9、底物
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