- ￥5800
- Proteintech
- 66914-1-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Cathepsin L protein
- 亚型：
IgG2b
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
- 靶点：
Cathepsin L
- 抗体英文名：
Cathepsin L Monoclonal antibody, PBS Only (Detector)
- 抗体名：
Cathepsin L Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
66914-1-PBS targets Cathepsin L as part of a matched antibody pair:
MP51216-1: 66914-2-PBS capture and 66914-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag27951 Product name: Recombinant human Cathepsin L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-333 aa of BC012612 Sequence: MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNVKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cathepsin L1 |
| 别名 | CTSL1, 1A12G8, Cathepsin L heavy chain, Cathepsin L light chain, cathepsin L1 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC012612 |
| 基因名称 | Cathepsin L |
| Gene ID (NCBI) | 1514 |
| RRID | AB_2882241 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07711 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)
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文献和实验IgM亚型的单抗。 不过需要注意的是Protein L填料的成本也非常昂贵。 除了上述介绍的纯化方法外，单抗还可以用抗原亲和纯化，其具体原理和操作和多抗的纯化完全一样，有兴趣的可以参见本站多克隆抗体纯化一节，这里不再废话。 5. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交
避光。（4）吸取上述醛化好的HRP溶液3ml，加入5mg IgG的碳酸盐缓冲液1ml，室温轻搅2-3小时，避光；加入5mg NaBH4 ，4℃放置3小时或过夜，或换用乙醇胺（2mol/L PH9.5）0.2ml，作用7小时。（5）再移入透析袋中，在0.02mol/L PH7.4 PBS中透析24小时，更换三次缓冲液。（6）用3000r/min离心30分钟，除去沉淀物。上清液再用半饱和硫酸铵盐析三次，沉淀用少许PBS溶解，透析或层析除盐，必要时进一步层析纯化。（7）、（8）步骤同程序一。2、碱性
.2ml H2O 177.80ml 二、操作方法 1、用包被液将猪瘟弱毒单抗纯化酶联抗原、猪瘟强毒单抗纯化酶联抗原各做100倍稀释，每孔包被100µl，4℃湿盒过夜。 2、次日取出，甩去孔内液体，3×3′冲洗。 3、将待检血清以PBS液做400倍稀释，每孔加100µl同时将猪瘟阳性血清、猪瘟阴性血清各做100倍稀释，于对照孔中加100μl.37℃温育1.5h～2h。 4、重复第二步，取出，甩去孔内液体，3×3′洗涤。 5、将兔抗猪IgG酶标抗体以PBS液做100倍稀释，每孔加100μ
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