- ￥2390 - 5520
- Proteintech
- 65221-1-Ig
- 2025年10月09日
- FC
- Rat
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
B7-H3/CD276
- 抗体英文名：
Anti-Mouse B7-H3/CD276 (MJ18)
- 抗体名：
Anti-Mouse B7-H3/CD276 (MJ18)
- 规格：
100ug/500ug
| 规格： | 100ug | 产品价格： | ￥2390.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥5520.0 |
经过测试的应用
| Positive FC detected in | mouse peritoneal macrophages |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 1 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65221-1-Ig targets B7-H3/CD276 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD276 antigen |
| 别名 | CD276, CD276 antigen, B7RP 2, B7-H3, B7h3 |
| GenBank蛋白编号 | BC019436 |
| 基因名称 | Cd276 |
| Gene ID (NCBI) | 102657 |
| RRID | AB_2935144 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8VE98 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD276 (also known as B7-H3) is a type I transmembrane protein expressed on many tissues and cell types. CD276 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, CD276 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
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文献和实验49e/CD29、CD49f/CD29、CD51/CD29、CD11a/CD18、CD11b/CD18、CD11c/CD18、CD41/CD61、CD51/CD61、CD49f/CD104、CD2、CD4、CD8、CD28、CD31、CD50、CD54、CD56、CD58、CD80、CD102、CD106、CD62L、CD62P、CD62E、CD15、CD15s、CD44等。 （2）B表示CD命名范围中属IGSF结构的分子，如CD79a、CD79b、CD1、CD
部分（α3），Poly IgR与多聚Ig，FcγRⅠ（CD64）、FcγRⅡ(CD32)、FcγRⅢ（CD16）与Ig Fc段，FcεRⅠ与IgE Fc段，FcαR（CD89）与IgA Fc段，CD28与B7/BB1（CD80）等之间的相互识别。 2.IGSF和integrin相互识别 如ICAM-1（CD54）、ICAM-2（CD102）、ICAM-3（CD50）与LFA-1（CD11a/CD18），VCAM-1（CD106）与VLA-4（CD49d/CD29)之间的相互作用。
Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.
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