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Cardinal Red™ Anti-Human CD45

(HI30) CR-65109
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  • ¥920 - 2310
  • Proteintech已认证
  • CR-65109
  • 2025年10月09日
  • FC
  • Mouse
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 亚型

      IgG1, kappa

    • 保存条件

      Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

    • 克隆性

      Monoclonal

    • 标记物

      Cardinal Red™ Fluorescent Dye

    • 适应物种

      human

    • 宿主

      Mouse

    • 应用范围

      FC

    • 靶点

      CD45

    • 抗体英文名

      Cardinal Red™ Anti-Human CD45 (HI30)

    • 抗体名

      Cardinal Red™ Anti-Human CD45 (HI30)

    • 规格

      25tests/100tests

    规格:25tests产品价格:¥920.0
    规格:100tests产品价格:¥2310.0

    经过测试的应用

    Positive FC detected inhuman PBMCs
    Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

    推荐稀释比

    应用推荐稀释比
    Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
    This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    CR-65109 targets CD45 in FC applications and shows reactivity with human samples.

    经测试应用 FC
    经测试反应性 human
    免疫原

    fusion protein

    宿主/亚型 Mouse / IgG1, kappa
    抗体类别 Monoclonal
    产品类型 Antibody
    全称 protein tyrosine phosphatase, receptor type, C
    别名 EC:3.1.3.48, GP180, HI30, L CA, LCA
    GenBank蛋白编号BC014239
    基因名称 CD45
    Gene ID (NCBI) 5788
    ENSEMBL Gene IDENSG00000081237
    RRIDAB_3084949
    偶联类型 Cardinal Red™ Fluorescent Dye
    最大激发/发射波长592 nm / 611 nm
    形式Liquid
    纯化方式Affinity purification
    UNIPROT IDP08575
    储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
    储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

    背景介绍

    CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

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    图标文献和实验
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    • [实验步骤] 由全血直接分选 CD3+ T 细胞

      : 由此步骤开始,流出的溶液包含目标细胞,请使用新的试剂管收集!首先,加入 1 ml 的 biotin 洗脱液,孵育 2 min。其次,加入 10 ml biotin 洗脱液,洗脱目标细胞。d) 再加入 10 ml biotin 洗脱液,洗下剩余的目标细胞。最后两步骤所收集的液体含分选出的目标细胞。(4) 结果分析以下流式图为从 7.5 ml 全血中,选出 CD3+ T 细胞的数据。选后细胞以 CD3-APC(OKT-3) 及 CD45-PE(HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞

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