- ￥5800
- Proteintech
- 65286-1-PBS
- 2026年04月17日
- FC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
IL-10
- 抗体英文名：
Anti-Human IL-10 (7B8G3), PBS Only
- 抗体名：
Anti-Human IL-10 (7B8G3), PBS Only
- 规格：
100ug
65286-1-Ig
产品信息
65286-1-PBS targets IL-10 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 | fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 10 |
| 别名 | IL-10,IL10, 7B8G3, CSIF, IL 10, IL10 |
| 计算分子量 | 21 kDa |
| GenBank蛋白编号 | BC104252 |
| 基因名称 | IL-10 |
| Gene ID (NCBI) | 3586 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P22301 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
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文献和实验background when used for immunocytochemistry, although we have found that this can be eliminated by both minimizing the spheroplasting process and preadsorbing primary and secondary antibodies in multiple incubations against fixed yeast cells suspended in PBS
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
Anti-GFP/BrdU In Situ Immunostaining
of 2 N HCl x30'. Wash 3x in PBS. Block in 1 mL 10% Horse serum, x30' at room temp. Wash 1x in PBS. Add 0.3 mL Biotinylated anti-BrdU (1:300, with 1% horse serum in PBS). Incubate at 4ºC o.n. or 2 hr. at room temp. Wash 3x in PBS.
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