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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human FMO1 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
FMO1
- 抗体英文名:
CoraLite® Plus 750-conjugated FMO1 Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 750-conjugated FMO1 Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive WB detected in | mouse liver tissue, human liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83156-3 targets FMO1 in WB applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag34267 Product name: Recombinant human FMO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-430 aa of NM_001282692 Sequence: LLKHIQFKTKVCSVTKCSDSAVSGQWEVVTMHEEKQESAIFDAVMVCTGFLTNPYLPLDSFPGINAFKGQYFHSRQYKHPDIFKDKRVLVIGMGNSGTDIAVEASHLAEKVFLSTTGGGWVISRIFDSGYPWDMVFMTRFQNMLRNSLPTPIVTWLMERKINNWLNHANYGLIPEDRTQLKEFVLNDELPGRIITGKVFIRPSIKEVKENSVIFNNTSKEEPIDIIVFATGYTFAFPFLDESVVKVEDGQASLYKYIFPAHLQKPTLAIIGLIKPLGSMIPTGETQARWAVRVLKGVNKLPPPSVMIEEINARKENKPSWFGLCYCKALQS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | flavin containing monooxygenase 1 |
| 别名 | Trimethylamine monooxygenase, Flavin-containing monooxygenase 1, Fetal hepatic flavin-containing monooxygenase 1, EC:1.14.13.148, Dimethylaniline monooxygenase [N-oxide-forming] 1 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 53-60 kDa |
| GenBank蛋白编号 | NM_001282692 |
| 基因名称 | FMO1 |
| Gene ID (NCBI) | 2326 |
| RRID | AB_3673773 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q01740 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Flavin-containing monooxygenase 1 (FMO1) belongs to the FMO family. FMO1 is an enzyme that catalyzes the oxygenation of a series of sulfur-containing and nitrogen-containing substrates, and thus is involved in a series of drugs and xenobiotics metabolism. FMO1 can regulate PPAR-α and mediate metabolic processes. FMO1 is expressed at relatively high levels in human fetal liver and also represents the major FMO enzyme in the adult human and rabbit intestine and kidney (PMID: 11809920, 30757917, 37553313). Human FMO1 has 2 isoforms with the molecular weights of 53 and 60 kDa. The observed molecular weight of FMO1 is 53 kDa, which might be the unmodified form of FMO1 (PMID: 9778310).
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
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体内后,通过糖苷键结合参与代谢,转换为葡萄糖醛酸轭合物或硫酸轭合物。所以β-受体激动剂的检测通常需要酶解步骤,β-葡萄糖醛酸酶是一种可以使葡萄糖醛酸苷键加水分解的酶,它可以把葡萄糖醛酸轭合物或硫酸轭合物的糖苷键切断,释放出游离态。目前,β-受体激动剂类药物残留的检测方法包括高效液相色谱法(HPLC)、气相色谱-质谱法(GC-MS)、液相色谱-质谱/质谱法(LC-MS/MS)、毛细管电泳电气化学检测法。下面介绍使用新型重组酶 IMCSzyme® β-葡萄糖醛酸酶与 MicroSep® DPX 枪头式分散固相
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