- ￥5800
- Proteintech
- 85875-1-PBS
- 2025年10月09日
- WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HK2 protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Hexokinase 2
- 抗体英文名：
Hexokinase 2 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Hexokinase 2 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
85875-1-PBS targets Hexokinase 2 as part of a matched antibody pair:
MP02173-3: 85875-3-PBS capture and 85875-1-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16895 Product name: Recombinant human HK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 568-917 aa of BC021116 Sequence: HGTGDELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDESILLKWTKGFKASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGFEDPHCEVGLIVGTGSNACYMEEMRNVELVEGEEGRMCVNMEWGAFGDNGCLDDFRTEFDVAVDELSLNPGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALLQVRAILQHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAAVVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQSEDGSGKGAALITAVACRIREAGQR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | hexokinase 2 |
| 别名 | HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2 |
| 计算分子量 | 102 kDa |
| 观测分子量 | 102-120 kDa |
| GenBank蛋白编号 | BC021116 |
| 基因名称 | Hexokinase 2 |
| Gene ID (NCBI) | 3099 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P52789 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).
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文献和实验μL。 封闭液：用PBS将试剂盒中的封闭存储液(Blocking stock solution R)稀释10倍，每孔200μL。 抗体稀释液：注意，只是用来稀释检测抗体和酶联亲和素。用PBS将试剂盒中的稀释存储液(Dilution buffer R)稀释10倍 检测抗体(Biotinylated detector antibody，Secondary antibody)：照U-Cytech说明书，加入双蒸水溶解。使用时，用抗体稀释液稀释100倍，每孔
50μL。 封闭液：用PBS将试剂盒中的封闭存储液（Blocking stock solution R）稀释10倍，每孔200μL。 抗体稀释液：注意，只是用来稀释检测抗体和酶联亲和素。用PBS将试剂盒中的稀释存储液（Dilution buffer R）稀释10倍 检测抗体（Biotinylated detector antibody，Secondary antibody）：照U-Cytech说明书，加入双蒸水溶解。使用时，用抗体稀释液稀释100倍，每孔100μL。 酶联亲和
以非那西丁为探针采用高效液相色谱法快速测定细胞色素P450 1A2的酶活性
acid). The flow rate was 2.0 ml・min-1, and the UV detector was set at 244 nm. Firstly, phenacetin was incubated with human CYP 450 1A2 enzyme in vitro at 37℃ and stopped by addition of 50 μl acetonitrile and centrifuged (10 000 g) for 3 min. Finally
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