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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human SLC6A12 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
Human
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
SLC6A12
- 抗体英文名:
CoraLite® Plus 750-conjugated SLC6A12 Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 750-conjugated SLC6A12 Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83195-5 targets SLC6A12 in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag18778 Product name: Recombinant human SLC6A12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 548-614 aa of BC126215 Sequence: VCVPLFVVITLLKTRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSPTREGLIAGEKETHL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 |
| 别名 | BGT 1, BGT1, FLJ38727, SLC6A12 |
| 计算分子量 | 614 aa, 69 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC126215 |
| 基因名称 | SLC6A12 |
| Gene ID (NCBI) | 6539 |
| RRID | AB_3673777 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P48065 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验,000g离心10分钟; (6) 转移上清液于分离柱中,室温下,10,000g离心1分钟; (7) 倒弃流出液,加入500μl HB Buffer,室温下,10,000g离心1分钟 (8) 倒弃流出液,加入750μl Wash Buffer,室温下,10,000g离心1分钟,倒弃流出液,重复此步骤一次; (9) 室温下,10,000g离心1分钟,以去除分离柱中残余的乙醇 (10) 将分离柱重新放入一个干净的1.5ml离心管中,加入30~50μl灭菌水,静置3-5分钟,定温
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
the collection tube for next step. 12. Place the HiBind® DNA column back into the collection tube and add 750ul of 80% ethanol. Centrifuge at > 8,000 x g for 30 seconds at room temperature. Discard the flow-through and re-use the collection tube. 13
BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
a single colony from a freshly streaked selective plate, and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20-24 hr at 37°C with vigorous shaking (~ 300 rpm). Use a flask
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