- ￥5800
- Proteintech
- 66855-1-PBS
- 2025年10月09日
- WB, IHC, Indirect ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-6R protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
WB, IHC, Indirect ELISA
- 靶点：
CD126/IL-6R alpha
- 抗体英文名：
CD126/IL-6R alpha Monoclonal antibody, PBS Only
- 抗体名：
CD126/IL-6R alpha Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
66855-1-PBS targets CD126/IL-6R alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag18263 Product name: Recombinant human IL-6R protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 22-371 aa of BC132684 Sequence: PRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLPTFLVAG |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 6 receptor |
| 别名 | CD126, IL-6R alpha, 2A5F4, IL 6R alpha, IL 6R 1 |
| 计算分子量 | 468 aa, 52 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC132684 |
| 基因名称 | IL-6R alpha |
| Gene ID (NCBI) | 3570 |
| RRID | AB_2882195 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P08887 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).
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文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
避光。（4）吸取上述醛化好的HRP溶液3ml，加入5mg IgG的碳酸盐缓冲液1ml，室温轻搅2-3小时，避光；加入5mg NaBH4 ，4℃放置3小时或过夜，或换用乙醇胺（2mol/L PH9.5）0.2ml，作用7小时。（5）再移入透析袋中，在0.02mol/L PH7.4 PBS中透析24小时，更换三次缓冲液。（6）用3000r/min离心30分钟，除去沉淀物。上清液再用半饱和硫酸铵盐析三次，沉淀用少许PBS溶解，透析或层析除盐，必要时进一步层析纯化。（7）、（8）步骤同程序一。2、碱性
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