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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
WB, Indirect ELISA
- 靶点:
BST2
- 抗体英文名:
Mouse BST2 Recombinant monoclonal antibody, PBS Only
- 抗体名:
Mouse BST2 Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
85614-4-PBS targets BST2 in WB, Indirect ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2669 Product name: Recombinant Mouse Bst2 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 52-152 aa of NM_198095.2 Sequence: TANSVACRDGLRAQAECRNTTHLLQRQLTRTQDSLLQAETQANSCNLTVVTLQESLEKKVSQALEQQARIKELENEVTKLNQELENLRIQKETSSTVQVNS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | bone marrow stromal cell antigen 2 |
| 别名 | Bone marrow stromal antigen 2, BST-2, CD317, HM1.24 antigen |
| 计算分子量 | 19 kDa |
| 观测分子量 | 30-36 kDa |
| GenBank蛋白编号 | NM_198095.2 |
| 基因名称 | Bst2 |
| Gene ID (NCBI) | 69550 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8R2Q8 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).
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文献和实验倍的pbs,这个问题也曾经困扰了我很久,因为当时要用0.01mol/L的 PBS,多方查证才证实,我们平时免疫组化用的是就0.01的pbs,也就是一倍的pbs, 0.1mol/L PBS即10倍PBS的配方如下:氯化钠 8.5g,十二水磷酸氢二钠2.85克(二水磷酸氢二钠1.13克),氯化钾0.2克,磷酸二氢钾0.27克,蒸馏水100毫升。 丁香园网友mouse778191的观点: 我用的是: 水:1000毫升 十二水磷酸氢二钠:6克 二水磷酸二氢钠:0.4克 氯化钠:9克 PH
1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞,只有少数是分泌预定特异性单克隆抗体的细胞,因此,必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法,筛选出能产生所需单克隆抗体的阳性杂交瘤细胞,并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后,及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。四、重组病毒鉴定 1、转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清,感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d,当1/3-1/2细胞漂浮时
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