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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human TGFB1I1 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, rat
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
HIC5
- 抗体英文名:
HIC5 Recombinant monoclonal antibody
- 抗体名:
HIC5 Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, rat colon tissue, SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85859-5-RR targets HIC5 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag0868 Product name: Recombinant human TGFB1I1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-230 aa of BC001830 Sequence: MPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAW |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transforming growth factor beta 1 induced transcript 1 |
| 别名 | Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein, TGFB1I1 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC001830 |
| 基因名称 | HIC5 |
| Gene ID (NCBI) | 7041 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O43294 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIC5 (Hydrogen Peroxide-Inducible Clone 5), also known as TGFB1I1, ARA55, or TSC-5. It was first identified in 1994 as a gene induced by transforming growth factor-β (TGF-β) or hydrogen peroxide (PMID: 36222304). HIC5 is a member of the Paxillin protein family and functions as a molecular adapter, delivering signals when the cellular adhesion environment changes. Given its role in cancer and fibrotic diseases, HIC5 is a potential therapeutic target. For example, targeting HIC5 or the GR tau2 region could modulate the set of genes regulated by GR, providing opportunities for intervention (PMID: 24591583).
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文献和实验ml)lml,再从鸡的翼下静脉取血0.5--1ml,取出后放入刻度离心管中,再加入2--3ml Alsver液,使总量为4--5ml,混匀并封口,置于4℃冰箱内可供一周内使用,实验时取贮备的鸡血细胞lml,加入4ml85%生理盐水,混匀后以1200rr/min离心5min,去上清液后,最后用0.6mol/L的甘露醇液(或0.6mol/L蔗糖溶液)混匀后以上述离心条件洗涤两次,最后用0.6mol/L的甘露醇液将鸡细胞稀释并调整成每毫升含2×105个。如采用传代培养的骨髓或腹水瘤细胞,可先用0.9%
是没有问题的. 2、表达重组蛋白时,细菌裂解方法都有哪些? 在表达重组蛋白时,诱导以后跑SDS-PAGE发现表达都很好,但是在裂解细胞时遇到问题。总是不能彻底裂解细胞。 首先我采用超声裂解,可是不管我如何超声总有部分细菌没有裂解。 我的超声条件如下:宁波新芝超声细胞破碎仪,功率400W,超5秒,间隔5秒,重复99次。然后显微镜观察,不彻底时再重复99次。菌体来自200 ml培养液,用20 ml PBS重悬。 然后我又尝试加溶菌酶,首先加至终浓度100 ug/ml,4C半小时菌液不变粘
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