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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
Albumin
- 抗体英文名:
Rat Albumin Recombinant monoclonal antibody, PBS Only
- 抗体名:
Rat Albumin Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
83697-5-PBS targets Albumin in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | albumin |
| 别名 | Alb, 240745E3, rat albumin, Serum albumin |
| 计算分子量 | 69 kDa |
| 观测分子量 | 69 kDa |
| 基因名称 | Alb |
| Gene ID (NCBI) | 24186 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P02770 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Albumin is a multi-functional protein that participates in the regulation of colloid osmotic pressure, transportation of endogenous ligands and drugs, and regulation of microvascular permeability. Albumin major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Human (HSA) and rat (RSA) serum albumins share similar characteristics of binding biologically active substances.
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文献和实验液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。四、重组病毒鉴定 1、转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清,感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d,当1/3-1/2细胞漂浮时
Fusion of Mouse, Rat, or Hamster Cell
sterile PBS. Take an aliquot for a cell and viability count. Fusion Procedure. Mix the spleen and myeloma cells together in a 50 ml tube at a ratio of 2:1-5
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents • Freshly removed or flash frozen rat liver • PBS •Homogenization Buffer •Homogenization Buffer containing 0.5 mM mM
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