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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
100ul
| 产品编号 | bs-40296G-IRDye800CW |
| 英文名称 | AffiniPure Goat Anti-Mouse IgG H&L(min X Hu, Bov, Hrs, Rb, Sw Sr Prot), IRDye 800CW conjugated |
| 中文名称 | IRDye 800cw标记的亲和纯化山羊抗小鼠IgG (H+L)(最小交叉反应:Hu, Bov, Hrs, Rb, Sw Sr Prot) |
| 英文别名 | IRDye® 800CW Goat anti-Mouse IgG H+L; |
| 中文别名 | 山羊抗小鼠IgG H&L (IRDye® 800CW)预吸附二抗; |
| 产品应用 | WB=1:10000-25000 Not yet tested in other applications. |
| 抗体来源 | Goat |
| 免疫原 | Native Mouse IgG |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 1.0 mg/ml |
| 储存液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IRDye800CW Conjugated Goat (polyclonal) Anti-Mouse IgG(H+L), Highly Cross AdsorbedProducer: Li-Cor(USA)Product Number: 926-32210Fluorophore: IRDye 800CW(MW: 1166)Excitation Wavelength: 778nm(in PBS)Emission Wacelegth: 795nm(in PBS)Fluorophore/Protein Ratio: 1.9 moles IRDye 800CW: 1mole IgGIRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of mouse IgG as well as the light chains common to most mouse immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, bovine, horse, rabbit and swine serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:10000-25000} |

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文献和实验( min X….Mouse, Rat Sr Prot )。 以下是圆括号中所使用的一些简称: Bov = Bovine Ck = Chicken Gt = Goat GP = Guinea Pig Sy Hms = Syrian Hamster Hrs = Horse Hu = Human Ms = Mouse Rb = Rabbit Shp
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
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