Sample:
Lane 1,4,7: Mouse Testis tissue lysates
Lane 2,5,8: Rat Testis tissue lysates
Lane 3,6,9: Human MCF-7 cell lysates
Primary: Anti-beta-Actin (bs-0061R) at 1/1:2000 dilution
Secondary: IRDye800CW Goat anti-Rabbit IgG (bs-40295G-IRDye8) at 1:50000~200000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
- ￥880
- Bioss
- bs-40295G-IRDye800CW
- 2025年10月10日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
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100ul
| 产品编号 | bs-40295G-IRDye800CW |
| 英文名称 | Goat Anti-Rabbit IgG H&L, IRDye 800cw conjugated |
| 中文名称 | IRDye 800cw羊抗兔IgG (H+L)(进口分装) |
| 英文别名 | IRDye® 800CW Goat anti-Rabbit IgG H&L; Immunoglobulin G; |
| 中文别名 | 山羊抗兔IgG H&L (IRDye® 800CW)预吸附二抗; ®IRDye 800cw 羊抗兔IgG (H+L); |
| 产品应用 | WB=1:10000-30000 Not yet tested in other applications. |
| 交叉反应 | Rabbit, Mouse |
| 抗体来源 | Goat |
| 免疫原 | Native Rabbit IgG |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 1.0 mg/ml |
| 储存液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IRDye800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross AdsorbedProducer: Li-Cor(USA)Product Number: 926-32211Fluorophore: IRDye 800CW(MW: 1166)Excitation Wavelength: 778nm(in PBS)Emission Wacelegth: 795nm(in PBS)Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgGIRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:10000-30000} |
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文献和实验该产品被引用文献
[IF={{ 7.3 }}] {Yaru Zhang. et al. Ozone exposure induced kidney damage in diabetic mice: The key role of lipid metabolism and water-electrolyte homeostasis. ENVIRON POLLUT. 2025 May;372:125963} {IF} {Mouse}
[IF={{ 6 }}] {Jiaxue Wang. et al. Vanillin Has Potent Antibacterial, Antioxidant, and Anti-Inflammatory Activities In Vitro and in Mouse Colitis Induced by Multidrug-Resistant Escherichia coli. ANTIOXIDANTS-BASEL. 2024 Dec;13(12):1544} {WB} {Mouse}
[IF={{ 5 }}] {Zhou Xianfei. et al. Hypoxia-induced autophagy in pancreatic cancer counteracts the cytotoxicity of CD8+ T cells by inhibiting the expression of MHC-I. GENES IMMUN. 2024 Dec;:1-9} {IF} {Human}
[IF={{ 4.7 }}] {Mufeng Li. et al. Anti-osteosarcoma activity of Corynoline via Src/JNK signaling-mediated cell cycle arrest and mitochondrial apoptosis. INT IMMUNOPHARMACOL. 2025 Feb;148:114126} {} {}
[IF={{ 4.4 }}] {Yuanxin Zhang. et al. Mechanism of total saponins of Ranunculus ternatus Thunb. in treatment of breast cancer based on liquid chromatography–mass spectrometry and network analysis. FRONT PHARMACOL. 2025 Apr;16:} {WB} {Human}
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Goat Anti-Rabbit IgG H&L, IRDye 800cw conjugated(bs-40295G-IRDye800CW)-100ul
￥880
