Gibco 货号 C11885500BT 是一款国产化的 DMEM 低糖基础培养基，凭借适配多种哺乳动物细胞的特性，广泛应用于科研领域的细胞培养实验，以下是其详细产品介绍：
- 核心配方与特性
- 关键组分：该培养基含 1000mg/L 的 D - 葡萄糖（低糖浓度），能适配对葡萄糖需求较低的细胞代谢；同时添加 L - 谷氨酰胺和 110mg/L 丙tong酸钠，前者可为细胞核酸和蛋白质合成提供原料，后者可作为细胞代谢的替代碳源，提升细胞在低营养环境下的存活率；含酚红作为 pH 指示剂，方便实验者直观观察培养基酸碱变化，及时判断细胞培养环境稳定性。此外，产品明确不含 HEPES 缓冲液，实验中若需增强体系缓冲能力，需额外按需添加。
- 核心优势：作为 Gibco 标准化生产的国产培养基，它延续了该品牌培养基配方的兼容性和稳定性，既能满足基础细胞培养的营养需求，又通过国产化生产控制了成本，性价比适合高校、科研院所等频繁开展细胞实验的场景。
- 规格与储存条件
- 规格：常见规格为 500ml / 瓶，该容量适配多数常规细胞培养实验规模，也便于实验过程中避免培养基反复开封导致的污染问题。
- 储存运输：运输过程可常温运输，到货后需置于 2 - 8℃冷藏保存；保存期间需防潮、避光、置于阴凉处，避免受热或吸潮后滋生细菌，导致培养基变质，影响细胞培养效果。
- 适用范围与实际应用
- 适配细胞类型：属于通用性基础培养基，可支持多种哺乳动物细胞生长，包括原代成纤维细胞、神经元、胶质细胞、人脐静脉内皮细胞（HUVEC）、平滑肌细胞等原代细胞，以及 HeLa、293、Cos - 7 等常见细胞系。
- 实际科研应用：已被多项科研实验采用，例如在人胚胎干细胞分化为间充质干细胞的诱导实验中，它被用作基础培养基，搭配胎牛血清、生长因子等添加物构建诱导体系；在小鼠胚肝细胞诱导 HepG2 人肝癌细胞分化的机制研究中，该培养基也用于 HepG2 细胞的基础培养，为后续探究细胞分化相关分子机制提供了稳定的细胞培养基础。
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Gibco™ DMEM, Low Glucose, Chinese Domestic Grade
Cat No.: C11885500BT
Brand: Gibco (Thermo Fisher Scientific)
Form: Liquid, sterile
Size: 500 mL/bottle
Origin: Chinese domestic production (equivalent to Gibco 11885-084)
Product Description
Gibco™ DMEM (Dulbecco’s Modified Eagle Medium) low-glucose medium is a widely used basal medium for mammalian cell culture. This formulation contains 1,000 mg/L D-glucose, L-glutamine, and 110 mg/L sodium pyruvate, without HEPES. It is manufactured under Gibco’s quality standards, with full QC validation, providing reliable performance at a cost-effective price.
Key Components
- With: Low glucose (1.0 g/L), L-glutamine, sodium pyruvate (110 mg/L), phenol red, sodium bicarbonate
- Without: HEPES, additional growth factors or proteins
- Glucose: 1000 mg/L (1 g/L)
- Buffer: Sodium bicarbonate (3.7 g/L); for 5% CO₂ incubator
- pH: 7.2–7.4 (equilibrated)
- Osmolality: 320–360 mOsm/kg
- Sterility: 0.22 μm filter-sterilized; endotoxin ≤0.2 EU/mL; mycoplasma-free
Features
- Low-glucose formulation: More physiologically relevant for cells that prefer slow, steady metabolism (e.g., primary cells, fibroblasts)Thermo Fisher Scientific.
- Pyruvate-supplemented: Supports energy metabolism and reduces oxidative stress in sensitive cells.
- Classic DMEM nutrition: 4× amino acids and vitamins compared to original Eagle’s MEM, supporting robust cell growthThermo Fisher Scientific.
- Domestic production: Strict Gibco QC, lot-to-lot consistency, shorter lead time, competitive pricing.
- Ready-to-use: Liquid format, no preparation needed; ideal for routine culture.
Recommended Cells & Applications
- Primary cells: Fibroblasts, neurons, glial cells, HUVEC, smooth muscle cellsThermo Fisher Scientific.
- Cell lines: HeLa, HEK293, COS-7, PC-12Thermo Fisher Scientific.
- Applications: Routine maintenance, primary cell culture, long-term culture, metabolic studies, low-proliferation cell models.
Quality & Storage
- Shelf life: 12 months (unopened, 2–8°C).
- Storage: 2–8°C, protected from light; do not freeze.
- Intended use: For research only. Not for human/animal therapy or clinical diagnostics.
Typical Citation
Cells were maintained in Gibco DMEM low-glucose medium (C11885500BT, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin at 37 °C in 5% CO₂.
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