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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现货
- 供应商:
上海然其生物科技有限公司
- 英文名:
DMEM
- 规格:
500ml/瓶
1. Product Description
2. Key Features & Advantages
- High Glucose Formulation: Contains 4500 mg/L D-glucose, ideal for rapidly proliferating cells (e.g., cancer cells, hybridomas).
- Complete Supplements: Formulated with L-glutamine (stable dipeptide form), phenol red (pH indicator), and HEPES (buffer system)
- Superior Quality:
- Raw materials undergo strict quality control.
- Endotoxin level < 1.0 EU/mL
- Produced in a GMP-compliant environment
- Ready-to-Use: Sterile filtered through a 0.1 μm membrane; no preparation required.
- Consistent Performance: Low batch-to-batch variation ensures reliable experimental results.
3. Typical Formulation (BL301A)
- Glucose: 4500 mg/L (High Glucose)
- L-Glutamine: 584 mg/L白鲨
- HEPES: Buffering agent (stabilizes pH)
- Phenol Red: pH indicator白鲨
- Sodium Pyruvate: None白鲨
- Sterility: 0.1 μm filtered, sterile白鲨
- pH: 7.0 – 7.4
- Endotoxin: < 1.0 EU/mL白鲨
4. Applications
- Cell Proliferation & Viability: Maintaining and expanding various mammalian cell lines.
- Primary Cell Culture: Suitable for many types of primary cells.
- Cancer Research: Ideal for culturing fast-growing tumor cell lines.
- Hybridoma & Cloning: Supports growth of hybridoma cells and clonal expansion.
- Transfection Studies: Compatible with DNA transfection protocols.
- Vaccine & Biological Production: Used in industrial manufacturing processes.
5. Storage & Stability
- Storage Conditions: 2°C to 8°C, protected from light
- Shelf Life: 12 months under recommended storage conditions
- Handling: Avoid repeated warming and cooling; protect from light during use.
6. Recommended Usage
- Supplement with 5–10% Fetal Bovine Serum (FBS) for optimal cell growth.
- Suitable for both adherent and suspension cell cultures.
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文献和实验《miR-377-3p 通过靶向 TLR4 调控高糖诱导的 HK-2 细胞上皮 - 间质转化》期刊:《中国病理生理杂志》, 2024引用:" 人肾小管上皮细胞 HK-2 接种于含 10% 胎牛血清的Biosharp 高糖 DMEM 培养基(BL301A)中,置于 37℃、5% CO₂培养箱中常规培养。"《芹菜素通过抑制 PI3K/Akt 信号通路缓解高糖诱导的 H9c2 心肌细胞损伤》期刊:《中药药理与临床》, 2023引用:"H9c2 细胞用含 10% 胎牛血清的Biosharp DMEM 高糖培养基培养,待细胞融合至 80%-90% 时进行传代。"
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