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| 规格： | >100次 | 产品价格： | ￥746.0 |
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| 规格： | >500次 | 产品价格： | ￥2998.0 |
DAF-FM DA即3-Amino,4-aminomethyl-2',7'-difluorescein, diacetate，也称DAF-FM diacetate或4-Amino, 5-aminomethyl-2', 7'- difluorescein, diacetate。DAF-FM DA是最·新一代用于一氧化氮定量检测的荧光探针，比以前比较常用的一氧化氮荧光检测探针DAF-2 diacetate有多方面的改进。首先，DAF-FM DA和DAF-2 diacetate相比，最后和一氧化氮反应形成的荧光产物受pH值的影响小，在pH值大于5.5时不受pH值的影响。其次，DAF-FM DA和DAF-2 diacetate相比，前者产生的荧光更加稳定，不容易淬灭，这样更加便于检测。另外，DAF-FM DA和DAF-2 diacetate相比，前者对一氧化氮的检测灵敏度更高，相同条件下检测灵敏度可以提高接近2倍，最低检测浓度可以达到3nM。
DAF-FM DA可以穿过细胞膜(cell-permeable)，进入细胞后可以被细胞内的酯酶催化形成不能穿过细胞膜的DAF-FM 。DAF-FM本身仅有很弱的荧光，但在和一氧化氮反应后可以产生强烈荧光，激发波长为495nm，发射波长为515nm。DAF-FM DA检测一氧化氮的机制可以参考上图。任何可以检测fluorescein的仪器，包括荧光显微镜、激光共聚焦显微镜、流式细胞仪、荧光分光光度计或荧光酶标仪都可以用于该荧光探针的检测。右图为DAF-FM在不同浓度一氧化氮存在时的发射荧光扫描图谱(fluorescence emission spectra)。
DAF-FM DA的分子量为496.4，分子式为C25H18F2N2O7，HPLC分析纯度大于98%。
本DAF-FM DA为溶解于DMSO的淡黄色溶液，浓度为5mM。
本荧光探针适合于检测细胞内的一氧化氮水平，可以进行实时检测。如果收集细胞后再装载探针，通常S包装至少可以检测100个样品，M包装至少可以检测500个样品。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| S0019S-1 | DAF-FM DA (NO荧光探针) 5mM | 20µl |
| S0019S-2 | DAF-FM DA稀释液 | 50ml |
| － | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| S0019M-1 | DAF-FM DA (NO荧光探针) 5mM | 100µl |
| S0019M-2 | DAF-FM DA稀释液 | 250ml |
| － | 说明书 | 1份 |
保存条件：
-20℃保存，DAF-FM DA需避光保存，一年有效。
注意事项：
BSA和酚红(phenol red)对本荧光探针的检测有干扰，需避免。
第一次使用时请分装成小包装后-20℃保存，以避免反复冻融。
荧光探针应随用随配。配制好的探针工作液应立即使用，不能以后再用。
DAF-FM DA在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水浴温育片刻至全部融解后使用。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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文献和实验该产品被引用文献
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Mol Neurobiol. 2015 Apr;51(2):718-28.(IF 4.5)
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J DIABETES COMPLICAT. 2015 Aug;29(6):747-54.(IF 2.781)
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A novel quinolinylmethyl substituted ethylenediamine compound exerts anti-cancer effects via stimulating the accumulation of reactive oxygen species and NO in hepatocellular carcinoma cells
Eur J Pharmacol. 2020 Oct 15;885:173497.doi: 10.1016/j.ejphar.2020.173497.(IF 3.263)
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TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT
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Naringenin ameliorates homocysteine induced endothelial damage via the AMPKα/Sirt1 pathway
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Design and Investigation of Penetrating Mechanism of Octaarginine-Modified Alginate Nanoparticles for Improving Intestinal Insulin Delivery
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ICG/l-Arginine Encapsulated PLGA Nanoparticle-Thermosensitive Hydrogel Hybrid Delivery System for Cascade Cancer Photodynamic-NO Therapy with Promoted Collagen Depletion in Tumor Tissues
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Cadmium exposure induces endothelial dysfunction via disturbing lipid metabolism in human microvascular endothelial cells
J Appl Toxicol. 2021 May;41(5):775-788.doi: 10.1002/jat.4115.(IF 2.997)
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[Mn(PaPy2Q)(NO)]ClO4, a Near-Infrared Light activated release of Nitric Oxide drug as a nitric oxide donor for therapy of human prostate cancer cells in vitro and in vivo
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Glutathione-Activated NO-/ROS-Generation Nanoparticles to Modulate the Tumor Hypoxic Microenvironment for Enhancing the Effect of HIFU-Combined Chemotherapy
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Glutathione-Responsive Biodegradable Nanoplatform with Endogenous Esterase-Triggered Nitric Oxide Release for Gas Therapy and Enhanced Chemotherapy
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Plant Cell Physiol. 2021 Jul 17;62(3):458-471.doi: 10.1093/pcp/pcab002.(IF 4.062)
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Synergistic Therapy Using Doxorubicin-Loading and Nitric Oxide-Generating Hollow Prussian Blue Nanoparticles with Photoacoustic Imaging Potential Against Breast Cancer
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Skimmed Bovine Milk-Derived Extracellular Vesicles Isolated via "Salting-Out": Characterizations and Potential Functions as Nanocarriers
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Nitrate-Functionalized poly(ε-Caprolactone) Small-Diameter Vascular Grafts Enhance Vascular Regeneration via Sustained Release of Nitric Oxide
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A study of lovastatin and L-arginine co-loaded PLGA nanomedicine for enhancing nitric oxide production and eNOS expression
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A novel bioactive polyurethane with controlled degradation and L-Arg release used as strong adhesive tissue patch for hemostasis and promoting wound healing
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Melatonin Attenuates Dasatinib-Aggravated Hypoxic Pulmonary Hypertension via Inhibiting Pulmonary Vascular Remodeling
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Cyclocarya paliurus triterpenoids attenuate glomerular endothelial injury in the diabetic rats via ROCK pathway
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Glucose-Responsive ZIF-8 Nanocomposites for Targeted Cancer Therapy through Combining Starvation with Stimulus-Responsive Nitric Oxide Synergistic Treatment
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Targeting Inhibition of Accumulation and Function of Myeloid-Derived Suppressor Cells by Artemisinin via PI3K/AKT, mTOR, and MAPK Pathways Enhances Anti-PD-L1 Immunotherapy in Melanoma and Liver Tumor
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Novel Nitric Oxide Donor Dinitroazetidine-Coumarin Hybrids as Potent Anti-Intrahepatic Cholangiocarcinoma Agents
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Heart-targeted amelioration of sepsis-induced myocardial dysfunction by microenvironment responsive nitric oxide nanogenerators in situ
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ELO2 Participates in the Regulation of Osmotic Stress Response by Modulating Nitric Oxide Accumulation in Arabidopsis
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Pentapeptide AYP from Isochrysis Zhanjiangensis Exhibits Antiangiogenic Activity in HT1080 Cells and HUVECs by Suppressing Migration and Invasion In Vitro
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Oridonin attenuates low shear stress-induced endothelial cell dysfunction and oxidative stress by activating the nuclear factor erythroid 2-related factor 2 pathway
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High-throughput probing macrophage-bacteria interactions at the single cell level with microdroplets
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Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis
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Dimethylarginine dimethylaminohydrolase 1 protects PM2.5 exposure-induced lung injury in mice by repressing inflammation and oxidative stress
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A core/shell nanogenerator achieving pH-responsive nitric oxide release for treatment of infected diabetic wounds
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Zinc-doped calcium silicate additive accelerates early angiogenesis and bone regeneration of calcium phosphate cement by double bioactive ions stimulation and immunoregulation
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Starch and chitosan-based antibacterial dressing for infected wound treatment via self-activated NO release strategy
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线粒体荧光探针信息大全 （Probes for Mitochondria）包括各种常用探针，如JC-1，JC-9，TMRM，TMRE等 Mitochondria are found in eukaryotic cells, where they make up as much as 10% of the cell volume. They are pleomorphic organelles with structural
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
线粒体荧光探针信息大全 （Probes for Mitochondria）包括各种常用探针，如JC-1，JC-9，TMRM，TMRE等 Mitochondria are found in eukaryotic cells, where they make up as much as 10% of the cell volume. They are pleomorphic organelles with structural variations depending on cell type
细胞状态下细胞器的形态学变化情况，此外还可通过光学切片即断层扫描技术进行三维重建，显示细胞器的空间关系及其变化。适用于线粒体的荧光探针较多，如Mitotracker、DA SPMI、DA SPEI、JC-1、罗丹明 123等。高尔基复合体常用的荧光探针有NBD 酰基鞘氨醇（ceramide）、BODIPY 酰基鞘氨醇。内质网主要用Dil、DiOC6(3)。溶酶体的荧光探针有DAMP、neutral red。 9． 标记抗体、配体等常用的荧光探针 共聚焦激光扫描显微镜不仅可用免疫荧光分析固定的细胞或组织
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