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- 湖北省武汉市
- Mouse Csn1s2a重组蛋白表达
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：Q02862
基因名：Csn1s2a
蛋白名：Alpha-S2-casein-like A
蛋白别名：
Alpha-S2-casein-like A, Casein alpha S2-like A, Gamma-casein, PP22
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Csn1s2a相关研究文献：
1. Cell-specific and shared regulatory elements control a multigene locus active in mammary and salivary glands. [37591874]
2. Increased transcriptional activity of milk-related genes following the active phase of experimental autoimmune encephalomyelitis and multiple sclerosis. [17785846]
3. High variability of milk protein genes in Bos indicus cattle breeds of Cameroon and Nigeria and characterization of a new alphas1-casein promoter allele. [15747725]
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文献和实验2. Increased transcriptional activity of milk-related genes following the active phase of experimental autoimmune encephalomyelitis and multiple sclerosis. [17785846]
3. High variability of milk protein genes in Bos indicus cattle breeds of Cameroon and Nigeria and characterization of a new alphas1-casein promoter allele. [15747725]
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
). Do not commence surgery until there is no reflex reaction to this test. Take the anesthetised mouse and lay it on it's belly on a petri dish lid, taking care to keep the airway clear by resting the teeth on the edge of the petri dish. This makes it easier
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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