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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Glutathione Reductase Rabbit mAb
- 抗体英文名:
Glutathione Reductase Rabbit mAb
- 应用范围:
WB
- 宿主:
Recombinant Rabbit
- 适应物种:
Human;Mouse;Rat
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 亚型:
Rabbit IgG
- 免疫原:
A synthetic peptide of human Glutathione Reductase
purification
Affinity Purified
applications
WB
appl_detail
WB: 1/1000;
other_names
GR; HEL-75; HEL-S-122m
calculated_mw
Calculated MW: 56 kDa; Observed MW: 51 kDa
formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
uniprot
P00390
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文献和实验一种利用还原型 NAD( P)将氧化型谷胱甘肽( GS- SG)催化反应成还原型( GSH)的酶 EC. 1. 6. 4. 2。在动植物组织、微生物、酵母中均可以见到。反应是不可逆的。一经透析便会失活,通过添加 Mg2 或 M2 n 使之活化。鼠肝脏的酶几乎只能利用 NADP作为补酶,但是在人的红血球的酶也利用 NAD进行作用。对胱氨酸和高胱氨酸均无作用。可从酵母中制取结晶物。植物组织中似乎可对脱氢酶和氧化酶的连结起作用。将谷胱甘肽还原酶 GS- SG、 NADP
Measurement of Reduced Glutathione Using High-Pressure Liquid Chromatography
As outlined in Chapter 8 , glutathione in the intact cell is maintained predominantly in its reduced form by the cytosolic enzyme, glutathione reductase. Cell lysis can lead to rapid oxidation to the oxidized form, GSSG, and degradation by γ
), making protons available to neutralize reactive oxygen species (Reaction 1 ). In vivo, reduction of GSSG is catalysed by glutathione reductase, efficiently regenerating high intracellular GSH levels (Reaction 2 )
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