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- 2025年09月10日
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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris
-throughput screening platforms. Once identified, antigen-binding domains on fragment antibodies or single-chain fragment antibodies (scFv) can be expressed and purified for further studies. In this chapter, we describe a method for high-level expression
For siliconization of glass reaction vessels Phenethylamine Sigma Aldrich 407267-100ML >99.5% Hydrophobic side-chain amine
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