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文献和实验35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells
(1 -7 ). Production of selected Abs in sufficient quantity for further evaluation often requires the transfer of their coding sequences to vectors specfiically designed for expression in eukaryotic cells. For Fab and/or immunoglobulin G (IgG) expression, these systems
Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool
the analysis of multiple rearranged immunoglobulin sequences. In this chapter, we demonstrate the use of the new Fab Analysis – a unique tool that is tailored for the analysis of both heavy and light chain sequences of a given antibody or collection
Construction of Large Nave Fab Libraries
primary (unselected) immune repertoire via cloning of Abs that recognize a variety of Ags (1 ,2 ). The rearranged V genes were amplified with the polymerase chain reaction (PCR) from B-cell mRNAs encoding immunoglobulin M (IgM) taken from nonimmunized
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