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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB;ICC/IF
- 靶点:
Uniprot:P25963
- 抗体英文名:
IKB alpha(Phospho-S32) Rabbit mAb
- 规格:
50ul/100ul
I-kappa-B-alpha antibody
IkappaBalpha antibody
IkB-alpha antibody
IKBA antibody
IKBA_HUMAN antibody
IKBalpha antibody
MAD 3 antibody
MAD3 antibody
Major histocompatibility complex enhancer-binding protein MAD3 antibody
NF kappa B inhibitor alpha antibody
NF-kappa-B inhibitor alpha antibody
NFKBI antibody
NFKBIA antibody
Nuclear factor of kappa light chain gene enhancer in B cells antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
计算分子量:36 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
图片:

Western blot analysis of Phospho-IKB alpha(S32) on Ags cell lysates using anti-Phospho-IKB alpha(S32) antibody at 1/1,000 dilution.
,

ICC staining Phospho-IKB alpha(S32) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining Phospho-IKB alpha(S32) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验【求助】矛盾的实验结果:IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达,结合文献,推理应该是IKb alpha下降,而NFKB升高 (IKb可磷酸化NFKB,使后者降低) 但现在我的结果是两者同时升高,请问可能的原因有哪些呢? 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况? erik NFKB是检测核内的吗?检测p65还是p50/ 吴佰霖 检测总蛋白
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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