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- 保存条件:
常温
- 保质期:
根据瓶身LOT号查询
- 英文名:
Sodium acetate buffer solution
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
126-96-5
- 规格:
100ML
属性
等级
Molecular Biology
质量水平
200
无菌性
0.2 μm filtered
表单
liquid
浓度
3 M
pH值(酸碱度)
5.2±0.1 (25 °C)
适用性
suitable for DNA precipitation
储存温度
room temp
SMILES字符串
[Na+].CC(O)=O.CC([O-])=O
InChI
1S/2C2H4O2.Na/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+1/p-1
InChI key
BHZOKUMUHVTPBX-UHFFFAOYSA-M
一般描述
应用
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文献和实验Intestinal and hepatic microbiota changes associated with chronic ethanol administration in mice.
Alcohol-induced liver disease is closely related to translocation of bacterial products and bacteria from the intestine to the liver. However, it is not known whether bacterial translocation to the liver depends on certain intestinal microbiota changes that would predispose bacteria to translocate to the liver. In this study, we investigated the microbiota in the jejunum, ileum, cecum, feces and liver of mice subjected to chronic ethanol feeding using a Lieber DeCarli diet model of chronic ethanol feeding for 8 weeks. We demonstrate that chronic ethanol administration changes alpha diversity in the ileum and the liver and leads to compositional changes especially in the ileum. This is largely driven by an increase in gram-negative phyla - the source of endotoxins. Moreover, gram-negative Prevotella not only increased in the mucus layer of the ileum but also in liver samples. These results suggest that bacterial translocation to the liver might be associated with microbiota changes in the distal gastrointestinal tract.
过硫酸胺 0.1 g ,加超纯水1.0 mL 溶解后,4 ℃ 保存,保存时间为 1 周。(-20 ℃长期保存) 30% 丙烯酰胺 溶于总体积为 50 mL 的水中,定容至 100 mL。室温保存于棕色瓶。 10× PBS(2L) NaOH 调 pH 值至 7.4,定容至 2 L。保存于室温。 还原型 5×SDS 上样缓冲液 混匀后,分装于 1.5 mL 离心管中,4 ℃ 可保存数周,-20 ℃ 可长期保存。 电泳液缓冲液 加蒸馏水溶解定容至 1000 mL 室温保存,1 次溶液可重复
1.毕赤酵母表达常用溶液及缓冲液的配制1.1 各种母液的配制10*YNB:(含有硫酸铵、无氨基酸的13.4%酵母基础氮源培养基) 4℃保存。34g酵母基础氮源培养基(无硫酸铵)+100g硫酸铵,溶于1000ml水中,过滤除菌。500*B:(0.02%生物素 Biotin) 4℃保存 保存期为1年。20mg的生物素溶于100ml水中,过滤除菌。100*H:(0.4%Histidine 组氨酸) 4℃保存 保存期为1年。400mg的L-组氨酸溶于100ml水中,(加热至50℃以促进溶解),过滤
经查表知pH5.8浓度为0.2M Na 2 HPO 4 8.0毫升,而0.2M Na 2 HPO 4 92.0毫升。依此可推论出配制100ml0.1M的磷酸缓冲液需要0.1M Na 2 HPO 4 8.0毫升,而0.1M Na 2 HPO 4 需要92.0毫升。 所以500ml 0.1M磷酸缓冲液需要Na 2 HPO 4 量为:
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