- 询价
- Signalway Antibody
- 12717
- 2025年09月22日
- WB;IHC;IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr928: VQ(p-Y)RD
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB;IHC;IF
- 靶点：
Uniprot：P54762
- 抗体英文名：
EPHB1 (Phospho-Tyr928) Antibody
- 规格：
50ul/100ul
计算分子量:106 kDa
配方:0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
应用详情:WB 1:500 - 1:2000; IHC 1:100 - 1:500; IF 1:100 - 1:500
图片:
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: at 1/300 dilution
Secondary: at 1/20000 dilution
Predicted band size: 106 kD
Observed band size: 121 kD
,
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human U87MG cell lysates
Lane 5: Human MCF-7 cell lysates
Lane 6: Human U-2 OS cell lysates
Primary: at 1/1000 dilution
Secondary: at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 130 kDa
,
Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37??for 20 min; Primary:1:200, overnight at 4°C
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
. Immunoblot using antibodies to phospho-Tyr279/216 can assess alterations in activity independent ofthe N-terminal phosphorylation, although phosphorylation atthis site is most likely to be constitutive (14) . 3. Immunoblot using antibodies to known
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