- 询价
- Signalway Antibody
- 12213
- 2025年09月22日
- WB ELISA
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from human p27 around the phosphorylation site of S10.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB ELISA
- 靶点：
Uniprot：P46527
- 抗体英文名：
p27 (Phospho-Ser10) Polyclonal Antibody
- 规格：
50ul/100ul
计算分子量:22kd
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western blotting: 1/500 - 1/2000
ELISA: 1/10000
Not yet tested in other applications
图片:
Western Blot analysis of COLO205 cells using Phospho-p27 (S10) Polyclonal Antibody
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文献和实验Polyclonal Antibody Production
When you have the required amount of blood (1-5 mL is sufficient for a pre-bleed�for later bleeds take 20 mL), remove the needle and apply pressure to the wound. When the bleeding has stopped (~10 seconds), sterilize the ear with ethanol
then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
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