- 询价
- Signalway Antibody
- 43335
- 2025年09月22日
- WB IHC
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length fusion protein of human TBP
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC
- 靶点：
Uniprot：P20226
- 抗体英文名：
TBP Antibody
- 规格：
50ul/100ul
计算分子量:38kd
配方:Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
应用详情:Western blotting: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
图片:
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-4: 231 cell￡?Human testis tissue￡?Hela and Hepg2 cell
Primary antibody: 1/400 dilution
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 seconds
,
Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43335 at dilution 1/20.
,
Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #43335 at dilution 1/20.
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文献和实验Western Blot of TBP from TBP-GST bacteria
Culture bacteria 1. Grow glycerol stock A13 (TBP-GST in BL21) in 10ml LB and 10μl ampicillin (1:1000) 2. Shake @ 37℃ ON Induce bacteria 3. Adjust culture with LB until ON culture OD [@ 600nm is ~0.6-0.8 4. Induce with 40mM IPTG (made fresh
Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System
Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as transcription. TATA-binding protein (TBP) is one example of a protein that elicits
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