- 询价
- Signalway Antibody
- HW100
- 2025年09月22日
- WB ELISA
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from human Histone H3 around the mono-methylation site of K10.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB ELISA
- 靶点：
Uniprot：P68431
- 抗体英文名：
Histone H3 (Mono-Methyl-Lys10) Polyclonal Antibody
- 规格：
50ul/100ul
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western blotting: 1/500 - 1/2000
ELISA: 1/20000
Not yet tested in other applications
图片:
Western Blot analysis of MCF-7 cells using Mono-Methyl-Histone H3 (K10) Polyclonal Antibody
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文献和实验in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
Reagents 100-bp DNA size ladder (Promega) Agarose Antisera (affinity-purified) These should be raised against histone peptides with mono-, di-, or trimethylation or acetylation at a specific lysine/arginine residue
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
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