- 询价
- Signalway Antibody
- 11987
- 2025年09月04日
- WB;IF;ELISA
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser286. AA range:256-305
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB;IF;ELISA
- 靶点：
Uniprot：O14757
- 抗体英文名：
Chk1 (Phospho-Ser286) Antibody
- 规格：
50ul/100ul
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:
WB 1:500 - 1:2000.
IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
图片:
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and NonPhosphopeptide (Phospho-right), using Chk1 (Phospho-Ser286) Antibody
,
Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser286) Antibody. The picture on the right is blocked with the phospho peptide.
,
Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.
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文献和实验Mammalian CHK1 is a Ser/Thr effector kinase that plays critical roles in the DNA damage-activated cell cycle checkpoint signaling pathway downstream of ATR (ATM and Rad3-related protein kinase). This chapter is focused on describing an assay
licanming 我的实验是用VEGF处理细胞后测定细胞PKC激活水平，拟采用Western blot的方法，用ECL法检测，这样分析PKC激活程度结果国际上认可吗? 我在Cell Signaling Technology上，查到 2261 Phospho-(Ser) PKC Substrate Antibody W IP E D 9371 Phospho-PKC (pan) (betaII Ser660) Antibody W IP
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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