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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic acetylated peptide corresponding to residues surrounding Lys36 of human histone H3.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:P68431
- 抗体英文名:
Histone H3(Acetyl-Lys36) Rabbit Polyclonal Antibody
- 规格:
50ul/100ul
配方:Purified Rabbit IgG, 1mg/ml in PBS pH7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. .
应用详情:Western blotting: 1:200~1:500
图片:

Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using #HW078 diluted at 1:2,000.
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文献和实验IHC IC2575Acetyl-Histone H2B (Lys12) AntibodyRabbitW IHC IC2571Acetyl-Histone H2B (Lys20) AntibodyRabbitW IP IHC IC2574Acetyl-Histone H2B (Lys5) AntibodyRabbitW IP IHC IC9675Acetyl-Histone H3 (Lys18) AntibodyRabbitW IHC IC9674Acetyl-Histone H3 (Lys23
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
结果分析的重要步骤和Trouble shooting的依据,因为非特异性的免疫沉淀难以避免。 一般的设置方法 1 未经免疫 沉淀的超声处理后样本( INPUT)。 2 加特异抗体来源动物的正常血清或无关抗体(MOCK)。 3 阳性对照引物的应用。 4 选择不与目的蛋白质结合的基因组 区域作对照。 如 阳性对照抗体 (Anti-acetyl-Histone H3) * 阴性对照抗体 (Normal Rabbit
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