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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB IHC
- 靶点:
Uniprot:P49715
- 抗体英文名:
C/EBP-α (Phospho-Ser21) Antibody
- 规格:
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
图片:

Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-α (phospho-Ser21) Antibody #11648.The lane on the right is treated with the antigen-specific peptide.
,

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-α (phospho-Ser21) antibody #11648 (left)or the same antibody preincubated with blocking peptide (right).
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
adhesion and migration.Neuroscience Letters''450''327–33 31.X Tang''X Zhou''K Zhou''et al.(2009) Dauricine inhibits u insulin-like growth factor-I-induced hypoxia inducible factor 1α protein…Acta Pharmacologica Sinica. 30 (5): 605–616
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