Hu CD18 BV605 L130 50ug

产品概述

Hu CD18 BV605 L130 50ug 为 BD Pharmingen 品牌流式抗体，货号 744552，靶向 CD18 (Integrin β2)，荧光标记 BV605，克隆 L130 (RUO)，规格 50 ug。适用于 Flow cytometry (Qualified) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The L130 monoclonal antibody specifically binds to CD18 which is also known as β2 integrin. CD18 is an ~90-95 kDa type I transmembrane glycoprotein that is encoded by ITGB2 . CD18 associates with the integrin α chains CD11a, CD11b, CD11c, or CD11d to form the LFA-1, Mac-1, p150,95, or αDβ2 heterodimers, respectively. CD18 is expressed on virtually all normal peripheral blood leucocytes. CD18 serves as a leucocyte adhesion receptor and signaling receptor. It is essential for many immune responses that require cell-to-cell contact, such as lymphocyte adhesion, natural killer (NK) cell and T-lymphocyte cytolysis, and T lymphocyte proliferation. This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting

核心参数

荧光染料信息

BV605 The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:BV605最多激发源:407 nm最多发射源:605 nm

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing ≤0.09% sodium azide.

参考文献

Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997. Lanier LL, Ruitenberg JJ, Phillips JH. Functional and biochemical analysis of CD16 antigen on natural killer cells and granulocytes.. J Immunol. 1988; 141(10):3478-85. (Clone-specific: Functional assay). Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995. Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-314. (Biology). Testi R, Phillips JH, Lanier LL. Constitutive expression of a phosphorylated activation antigen (Leu 23) by CD3bright human thymocytes.. J Immunol. 1988; 141(8):2557-63. (Immunogen: Functional assay, Immunoprecipitation). Uciechowski P, Schmidt RE. Cluster report: CD18. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:597-598.