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- 文献和实验
- 技术资料
- 供应商:
BD Pharmingen
- 级别:
RUO
- 克隆性:
单克隆
- 抗原来源:
Human
- 标记物:
RY610
- 宿主:
Mouse
- 适应物种:
Human
- 免疫原:
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
- 亚型:
Mouse BALB/c IgG1, κ
- 形态:
液体
- 应用范围:
Flow Cytometry (FCM)
- 保存条件:
2-8℃避光保存
- 规格:
25 Tests
产品概述
Hu CD3 RY610 UCHT1 25Tst 为 BD Pharmingen 品牌流式抗体,货号 571135,荧光标记 RY610,克隆 UCHT1 (also known as UCHT-1; UCHT 1) (RUO),规格 25 Tests。适用于 Flow cytometry (Routinely Tested) 检测,仅供科研使用(RUO)。
靶点与抗体说明
The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.
核心参数
| 货号 | 571135 |
| 品牌 | BD Pharmingen |
| 克隆号 | 克隆 UCHT1 (also known as UCHT-1; UCHT 1) (RUO) |
| 宿主 | Mouse |
| 同种型 | Mouse BALB/c IgG1, κ |
| 荧光标记 | RY610 |
| 反应种属 | Human |
| 验证应用 | Flow cytometry (Routinely Tested) |
| 推荐用量 | 5 µl/test |
| 免疫原 | Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor |
| 规格 | 25 Tests |
| 存储缓冲液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
荧光染料信息
RY610 The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594. 格式:RY610最多激发源:557 nm最多发射源:610 nm
保存与运输
建议 2-8℃ 避光保存,请勿冷冻,避免反复冻融。 存储缓冲液:Aqueous buffered solution containing ≤0.09% sodium azide.
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文献和实验Elabscience流式克隆号选对不踩坑:6 大场景一次说清!
号选择的关键要点,帮助实验者在每个环节都能选对克隆号,让流式实验不再“翻车”。 场景一:活细胞表面抗原检测——必须选择识别胞外区构象表位的克隆号 活细胞表面抗原检测是流式基础免疫分型最常见的一类应用,其核心原则是:抗体必须在细胞膜保持完整、蛋白保持天然构象的条件下与靶标结合。因此,所选克隆号必须能够识别目标抗原上的胞外区构象表位,也就是由蛋白质三维折叠形成的、位于细胞外区域的空间结构。 例如T细胞的分析检测中,CD3抗体的不同克隆号存在较大差异,如OKT-3识别T细胞受体复合物的ε链,UCHT
of lung (AJSP 2002;26:715) Positive staining (normal): T cells (25-35% of mature peripheral T cells, most cytotoxic T cells, CD4/CD8+ thymocytes); NK cells (30%-which are also CD3 negative); cortical thymocytes (70-80%), epidermotrophic lymphocytes
Intracellular Cytokine Staining Protocol
+ CD25 BC96 14-0259 Natural T, NK+ CD56 MEM188 14-0569 small subset, CD3+CD56+ B
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