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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20° C,2 years.
- 保质期:
见优宁维官网
- 英文名:
Calcein-AM
- 库存:
999
- 供应商:
Absin
- 规格:
1mg
产品名:
Calcein-AM品牌:
Absin货号:
abs42014734保持条件:
Store at -20° C,2 years.浓度:
见优宁维官网免疫原:
见优宁维官网抗原名称:
见优宁维官网应用:
A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule使用方法:
other产品描述:
Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein , which is an extremely fluorescent and negatively charged molecule. The probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein as a cytosolic fluorophore. Has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition.
爱必信品牌介绍:
爱必信(上海)生物科技有限公司是一家从事生物学试剂及耗材的高新技术企业。公司秉承“为生命科学创造便利!为客户创造价值!为员工创造幸福人生!”的发展理念,拥有专业的产品研发质检团队、市场营销团队及先进仓储物流等管理团队。荣获2021年度上海市“专精特新”企业称号。
自营品牌Absin®,主要经营产品有:生化试剂(常用生化试剂、生物学实验常用试剂、荧光染料、激动剂/抑制剂,植物提取物)、细胞生物学(细胞培养产品、血清、基质胶、添加剂、检测试剂盒)、免疫学产品、分子生物学产品、实验室仪器/耗材、产品定制和检测服务。
Absin还提供一些独有的特色产品:霍乱毒素B亚单位(CTB)、 鸡胚提取物(CEE)、 牛脑垂体提取物、 呼吸爆发试剂盒、 多色免疫组化试剂盒、 mRNA原料等在业界深受客户喜欢。
Absin秉承踏实,务实,求精和不断创新的精神为中国科学家提供优质产品,努力打造属于中国自己的生物试剂品牌,为广大科学家提供更全面更丰富更优质的产品。

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文献和实验。 超过一定大小的癌细胞球接受了不同剂量的三种抗癌药物之一(顺铂、紫杉醇或 5-FU)或对照品的处理。处理 24 小时后,在所有孔中加入 Hoechst 33342、碘化丙啶(PI)和 Calcein-AM 染料。Hoechst 33342、PI 和 Calcein-AM 可分别染色细胞球内所有细胞的细胞核、凋亡细胞的细胞核以及活细胞。染色后,我们用激光扫描显微镜采集了癌症细胞球的图像数据。 2.三维癌症细胞球内细胞的图像采集 我们使用了 FLUOVIEW FV3000 激光扫描共聚
LIVE/DEAD® Violet Viability/Vitality Kit
than its parent compound. The calcein violet AM ester is colorless and non fluorescent until hydrolyzed. The polyanionic dye calcein violet is well retained within live cells, producing an intense uniform violet fluorescence in live cells (ex/em 400/452 nm
Primary Neuron Viability Assay
antibody (N-4142; Sigma, St. Louis, MO) demonstrated that approximately 80-90% of the cells present were neurons. Neuronal viability was assessed with the fluorescent viability dye, calcein-AM (Molecular Probes, Eugene, OR. Briefly, neuronal cultures
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