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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C, protect from light
- 英文名：
Calcein acetoxymethyl ester
- 库存：
货期：14天
- 供应商：
MedChemExpress LLC
- CAS号：
148504-34-1
- 规格：
100 μg（2 mg/mL * 50 μL in DMSO）/500 μg（2 mg/mL * 250 μL in DMSO）
| 规格： | 100 μg（2 mg/mL * 50 μL in DMSO） | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 500 μg（2 mg/mL * 250 μL in DMSO） | 产品价格： | ￥2500.0 |
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Calcein-AM
CAS No. : 148504-34-1
MCE 国际站：Calcein-AM
产品活性：Calcein AM，中文名为该黄绿素乙酰氧基甲指，具细胞膜渗透性，可轻易进入到细胞内，Calcein AM 身并无荧光，进入细胞后被细胞中内源性酯酶水解生成具有强负电荷的不能通透细胞膜的极性分子钙黄绿素 (Calcein)，从而被滞留在细胞内。而 Calcein 可发出强绿色荧光，因此常与 Propidium Iodide 用于细胞活力/毒力检测，激发/发射波长：494/515 nm。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: General Protocol
1.Preparation of Calcein AM working solution
Preparation of 5 mM stock solution with DMSO.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Calcein AM working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells（6-well plate）
a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 30-45 minutes.
c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or fluorescence microplate readers.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-45 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or fluorescence microplate readers.
Storage
-20°C
Protect from light
Precautions
1.Please adjust the concentration of Calcein AM working solution according to the actual situation.
2.This product is for R&D use only, not for drug, household, or other uses.
3.For your safety and health, please wear a lab coat and disposable gloves to operate.
In Vivo: Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability.
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文献和实验。 超过一定大小的癌细胞球接受了不同剂量的三种抗癌药物之一（顺铂、紫杉醇或 5-FU）或对照品的处理。处理 24 小时后，在所有孔中加入 Hoechst 33342、碘化丙啶（PI）和 Calcein-AM 染料。Hoechst 33342、PI 和 Calcein-AM 可分别染色细胞球内所有细胞的细胞核、凋亡细胞的细胞核以及活细胞。染色后，我们用激光扫描显微镜采集了癌症细胞球的图像数据。 2.三维癌症细胞球内细胞的图像采集 我们使用了 FLUOVIEW FV3000 激光扫描共聚
LIVE/DEAD® Violet Viability/Vitality Kit
than its parent compound. The calcein violet AM ester is colorless and non fluorescent until hydrolyzed. The polyanionic dye calcein violet is well retained within live cells, producing an intense uniform violet fluorescence in live cells (ex/em 400/452 nm
Primary Neuron Viability Assay
antibody (N-4142; Sigma, St. Louis, MO) demonstrated that approximately 80-90% of the cells present were neurons. Neuronal viability was assessed with the fluorescent viability dye, calcein-AM (Molecular Probes, Eugene, OR. Briefly, neuronal cultures
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