- ￥700 - 1700
- Solarbio|ActivAb
- 北京
- K011542M
- 2025年08月22日
- FCM
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Raised against granulocytes of mouse origin
- 亚型：
IgG2b κ
- 保存条件：
Store at 2-8℃ in dark.Do not freeze.
- 克隆性：
Monoclonal Antibody
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，Solar Fluor 350，Solar Fluor 405，Solar Fluor 488，Solar Fluor 532，Solar Fluor 555，Solar Fluor 568，Solar Fluor 594，Solar Fluor 633，Solar Fluor 640，Solar Fluor 647，Solar Fluor 660，Solar Fluor 680，Solar Fluor 750
- 适应物种：
Mouse
- 抗原来源：
Raised against granulocytes of mouse origin
- 目录编号：
546644
- 级别：
实验级别
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rat
- 应用范围：
FCM
- 浓度：
FCM 1×10^6 cells+0.06ug.
- 靶点：
Ly-6G/Ly-6C
- 抗体英文名：
Anti-Ly-6G/Ly-6C(PE) Monoclonal Antibody
- 抗体名：
Ly-6G/Ly-6C抗体
- 规格：
100ug/25ug
| 规格： | 100ug | 产品价格： | ￥1700.0 |
|---|---|---|---|
| 规格： | 25ug | 产品价格： | ￥700.0 |
| 基本信息 | |
| 英文名称 | Anti-Ly-6G/Ly-6C(PE) Monoclonal Antibody |
| 中文名称 | Ly-6G/Ly-6C抗体 |
| 宿主 | Rat |
| 克隆类型 | Monoclonal Antibody |
| 亚型 | IgG2b κ |
| 交叉反应 | Mouse |
| 应用 | FCM |
| 稀释比例 | FCM 1×10^6 cells+0.06ug. |
| 密度 | |
| 靶标 | Ly-6G/Ly-6C |
| 别名 | Gr-1; Gr1; Ly 6G; Ly-6G.1; Ly6G; lymphocyte antigen 6 complex locus G; Lymphocyte antigen 6G. |
| 背景说明 | Ly-6G (lymphocyte antigen 6 complex， locus G)， also known as Gr-1， is a 21-25 kDa， glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469). |
| Swiss Prot | P35461 |
| Gene ID | 546644 |
| 亚细胞定位 | Cell membrane |
| 免疫原 | Raised against granulocytes of mouse origin |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | PBS with 0.09% sodium azide and 0.5% BSA， pH 7.3. |
| 储存条件 | Store at 2-8℃ in dark.Do not freeze. |
| 偶联物 | PE |
| 单位 | PE |
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文献和实验D Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A D8 IgG2b, 09821D Eryth: Ter-119 IgG2b, 09081A Sca-1 PE-IgG2a (E31-161.7) 01835B PE-IgG2a Isotype control, 11025
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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