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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein of human GAPDH
- 亚型:
IgG
- 保存条件:
Store at -20℃. Avoid freeze/thaw cycles.
- 克隆性:
Polyclonal Antibody
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,Solar Fluor 350,Solar Fluor 405,Solar Fluor 488,Solar Fluor 532,Solar Fluor 555,Solar Fluor 568,Solar Fluor 594,Solar Fluor 633,Solar Fluor 640,Solar Fluor 647,Solar Fluor 660,Solar Fluor 680,Solar Fluor 750
- 适应物种:
Rat;Monkey;Human;Mouse
- 抗原来源:
Recombinant protein of human GAPDH
- 目录编号:
2597
- 级别:
实验级别
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 宿主:
Rabbit
- 应用范围:
IP;WB;IHC;IF;ELISA
- 浓度:
WB 1:8000-12000. IHC 1:50-200. IF 1:50-200. IP 1:50-500. ELISA 1:3000-30000.
- 靶点:
GAPDH
- 抗体英文名:
Anti-GAPDH Polyclonal Antibody
- 抗体名:
甘油醛-3-磷酸脱氢酶抗体
- 规格:
100ul/50ul
| 规格: | 100ul | 产品价格: | ¥780.0 |
|---|---|---|---|
| 规格: | 50ul | 产品价格: | ¥480.0 |
| 基本信息 | |
| 英文名称 | Anti-GAPDH Polyclonal Antibody |
| 中文名称 | 甘油醛-3-磷酸脱氢酶抗体 |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Rat;Monkey;Human;Mouse |
| 应用 | IP;WB;IHC;IF;ELISA |
| 稀释比例 | WB 1:8000-12000. IHC 1:50-200. IF 1:50-200. IP 1:50-500. ELISA 1:3000-30000. |
| 密度 | |
| 靶标 | GAPDH |
| 别名 | GAPD,CDABP0047,OK/SW-cl.12,G3PD,HEL-S-162eP |
| 背景说明 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, thereversible oxidative phosphorylation of glyceraldehyde-3-phosphatein the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. |
| Swiss Prot | P04406 |
| Gene ID | 2597 |
| 亚细胞定位 | Cytoplasm Cytosol cytoskeleton Nucleus Membrane |
| 免疫原 | Recombinant protein of human GAPDH |
| Public Immunogen Range | 1-336aa |
| 理论分子量 | 32/36kDa |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | PBS with 50% Glycerol,0.03% Proclin300,0.5% BSA,pH 7.3. |
| 储存条件 | Store at -20℃. Avoid freeze/thaw cycles. |
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Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
,但是进口品牌如Abcam、Millipore、CST等价格偏高,一般在¥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体,分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody (货号E021010 )是小鼠源的单抗,其效价很高,厂商推荐的WB起始稀释比率为1:1000,实际应用过程中,有使用1:10000或更高的报告,远远高于此稀释比率,说明其免疫效价很高,另外,其检测种属也很多,包括人、牛、猪、小鼠
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
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