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- 文献和实验
- 技术资料
- 抗体名:
Mouse anti-GSK3β Monoclonal mAb
- 抗体英文名:
Mouse anti-GSK3β Monoclonal Antibody
- 靶点:
见优宁维官网
- 浓度:
见优宁维官网
- 应用范围:
WB: 1:500-1:1000, IHC: 1:50-1:100, FCM: 1:50-1:100
- 宿主:
Mouse
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
Unlabeled
- 克隆性:
见优宁维官网
- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 免疫原:
Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.
- 规格:
100uL
别名:鼠抗GSK3β单克隆抗体
免疫原:Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.
应用:WB: 1:500-1:1000, IHC: 1:50-1:100, FCM: 1:50-1:100
保持条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
使用方法:WB,IHC,FlowCytometry
标记:Unlabeled
宿主:Mouse
反应种属:Human,Mouse,Rat
克隆性:见优宁维官网
产品描述:见优宁维官网
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文献和实验Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII
Diego, CA); GSK-3α (Upstate, LakePlacid, NY); and b-actin (Sigma, St. Louis, MO). 7. Secondary antibodies: anti-mouse and anti-rabbit IgG conjugatedto horseradish peroxidase (Santa Cruz Biotechnology,Santa Cruz, CA). 8. Enhanced chemiluminescent
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
Assays (See Note 9) 1. Pre-incubate anti-GSK3β (5 μg) monoclonal antibody withprotein G-Sepharose (400 μL of a 50% slurry) in kinasebuffer at 4°C for 1 h to conjugate the antibody to the proteinG-Sepharose beads. 2. Centrifuge the beads at 6,000× g
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