- $265
- Ybscience
- Yb--3168
- 美国/德国/中国
- 2025年07月08日
- WB ICC/IF IP
- Mouse
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
GSK3 β Mouse mAb
- 抗体英文名：
GSK3 β Mouse mAb
- 靶点：
详见说明书
- 浓度：
1.0mg/ml
- 应用范围：
WB ICC/IF IP
- 宿主：
Mouse
- 供应商：
上海钰博生物科技有限公司
- 库存：
大量
- 目录编号：
3168
- 抗原来源：
Mouse
- 保质期：
1年
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20°C
- 形态：
液体或冻干粉
- 亚型：
详见说明书
- 免疫原：
详见说明书
- 规格：
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--3168
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 46KDa
- UniProt : P49841
-
- Isotype : Mouse IgG2a
- Clonality : Monoclonal
-
- Price : $265/100μg
- Availability : 3-4 days
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3beta isoform;
Application:
WB ICC/IF IP
Reactivity:
Human,Mouse
Dilution:
WB 1:1000 ICC/IF 1:200 IP
 |
Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3β mouse mAb(1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa. |
 |
Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using GSK-3β mouse mAb (dilution 1:200). |
 |
Immunoprecipitation analysis of Hela cell lysates using GSK-3β.GSK-3β Rabbit mAb was used for the western blot analysis (1:1000 diluted). |
Purification:
Affinity-chromatography
Specificity:
GSK3 β Mouse mAb detects endogenous levels of total GSK3 β
Immunogen:
Purified recombinant GSK-3β protein fragments expressed in E.coli.
Description:
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验最近在做PI3K通路，查了不少文献，有的说糖尿病模型gsk增加，p-gsk减少，有的说gsk不变，p-gsk增加，本人做了好久，p-gsk3α/β条带一直很淡，很是纠结，想咨询下朋友们，我应该研究α还是β，谢谢了 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你，都可以成为师兄的好伙伴 师兄微信号：shixiongcoming
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
Staining Whole Mouse Embryos for β-Galactosidase (lacZ ) Activity Andras Nagy , Marina Gertsenstein , Kristina Vintersten , and Richard Behringer This protocol was adapted from "Techniques for Visualizing Gene Products, Cells, Tissues
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