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- 文献和实验
- 技术资料
- 抗体名:
Rabbit anti-CREB-1 Polyclonal mAb
- 抗体英文名:
Rabbit anti-CREB-1 Polyclonal Antibody
- 靶点:
见优宁维官网
- 浓度:
1mg/mL
- 应用范围:
WB: 1:500-2000, FCM: 1ug/Test
- 宿主:
Rabbit
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
Unlabeled
- 克隆性:
见优宁维官网
- 保存条件:
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
- 免疫原:
KLH conjugated synthetic peptide derived from human CREB-1
- 规格:
50uL/100uL
| 规格: | 50uL | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100uL | 产品价格: | ¥2300.0 |
别名:兔抗CREB-1多克隆抗体
免疫原:KLH conjugated synthetic peptide derived from human CREB-1
应用:WB: 1:500-2000, FCM: 1ug/Test
保持条件:Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
使用方法:WB,FlowCytometry,ELISA
标记:Unlabeled
宿主:Rabbit
反应种属:Human,Mouse,Rat,
克隆性:见优宁维官网
产品描述:见优宁维官网
爱必信品牌介绍:

更多详情请了解优宁维网站》》
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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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