- ￥1500 - 2300
- Absin
- abs147992
- 上海
- 2025年08月21日
- WB: 1:500-1:1000, IHC: 1:100-1:200
- Rabbit
- Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rabbit anti-CREB Polyclonal mAb
- 抗体英文名：
Rabbit anti-CREB Polyclonal Antibody
- 靶点：
见优宁维官网
- 浓度：
见优宁维官网
- 应用范围：
WB: 1:500-1:1000, IHC: 1:100-1:200
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep, Zebrafish
- 保质期：
见优宁维官网
- 抗原来源：
见优宁维官网
- 级别：
优级
- 库存：
999
- 供应商：
Absin
- 标记物：
Unlabeled
- 克隆性：
见优宁维官网
- 保存条件：
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CREB. The exact sequence is proprietary.
- 规格：
100uL/50uL
| 规格： | 100uL | 产品价格： | ￥2300.0 |
|---|---|---|---|
| 规格： | 50uL | 产品价格： | ￥1500.0 |
别名：兔抗CREB多克隆抗体
免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CREB. The exact sequence is proprietary.
应用：WB: 1:500-1:1000, IHC: 1:100-1:200
保持条件：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
使用方法：WB,IHC
标记：Unlabeled
宿主：Rabbit
反应种属：Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep, Zebrafish
克隆性：见优宁维官网
产品描述：
Rabbit polyclonal antibody to CREB.
爱必信品牌介绍：
更多详情请了解优宁维网站》》
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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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