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- 文献和实验
- 技术资料
- 库存：
999
- 英文名：
Percoll 细胞分离液
- CAS号：
/
- 保质期：
见瓶身
- 供应商：
麦格生物
- 保存条件：
见瓶身
- 规格：
1L
A low-viscosity, non-toxic medium suitable for density gradient centrifugation of cells, viruses and subcellular particles.
Composed of colloidal silica coated with polyvinylpyrrolidone (PVP), which gives low osmolality and low viscosity.
For separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability and morphological integrity.
Non-toxic to cells.
Adjustable to physiological ionic strength and pH.
Gradients can either be pre-formed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor.
Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g / mL.
Quick and easy
Percoll is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 × g). The medium is available in easy-to-open, resealable 250 mL and 1 L bottles.
Other attributes
Percoll consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll is non-toxic, almost chemically inert and does not adhere to membranes. Percoll gradients can be formed within the density range of 1.0 to 1.3 g/mL, and are iso-osmotic throughout.
Percoll can be stored unopened at room temperature for five years. At -20°C, it can only be stored for up to six months.
If stored at -20°C, gradients form upon thawing, necessitating a mixing of the bottle before use. Preformed gradients can be stored for weeks without a change in gradient shape, provided that the gradient remains unfrozen.
Percoll can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If pH drops below 5.5, gelling may occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures.
Undiluted Percoll can be resterilized by autoclaving for 30 min at 120°C.
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文献和实验。 Percoll浓度(％) 70 60 50 40 30 20 比重g／ml 1.090 1.077 1.067 1.056 1.043 1.031 2. 不连续密度梯度Percoll层的制备: 先将试管壁用牛血清湿润，除去多余血清，这种预处理可使逐层叠加的Percoll液
液界 liquid junction 指二种浓度或不同组成的电解质的接触面。液界可简单地把比重轻的溶液由重叠在重液之上而形成，但随着时间的进行，由于扩散或对流等可多少出现液体的混合。为防止混合可采取放置多孔质的膜以延缓扩散，或不断流动液体更新液界等方法。在液界上可产生液界电位（ liquid junction potential， EL ）。比较简单的是在浓度不同的同一种一价的电解质溶液间或浓度相同并且具有共同的离子的不同的电解质溶液间的情况下，液界电位的大小与液界的混合
试剂 1. 小牛血清 2. Percoll细胞分离液 3. 8.5％NaCl 4. 1.5MPBS 实验设备 1. 高速冷冻离心机(3K-30) 2. 4℃、-20℃冰箱 3. 长针头注射器 4. 1.5mL和10mL离心管 实验材料 PBMC细胞 实验步骤 1. 不同
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