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- 2026年01月13日
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Use Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC columns to provide the reproducibility, resolution, and robustness needed for protein characterization. These columns are ideal for charge variant analysis and recommended for use with therapeutic proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), and biosimilar proteins.
Designed with bio-inert materials to reduce secondary interactions, our ProPac 3R SCX columns deliver the performance you need over a broad range of pH's, temperatures, and mobile phase compositions with excellent recovery and low carryover.
Suitable for use with mass spectrometry (MS).
Maximizing Reproducibility, Resolution, and Robustness
The ProPac 3R SCX 3 μm HPLC column is a strong cation-exchanger with a sulfonic acid functional group, suitable for use in biomolecule applications. These specialized ion-exchange chromatography columns provide qualitative and quantitative evaluation of charge variant analysis to biopharmaceutical scientists who need reproducible resolving capabilities when identifying and quantifying charge variant profiles of proteins.
Our ProPac 3R SCX columns offer superior performance for characterization of proteins including monoclonal antibodies (mAbs), therapeutic proteins, antibody drug conjugates (ADCs), and biosimilar proteins.
Column Features:
- Ideal for characterization and quality control assessment of closely-related protein variants
- Unequaled high-resolution separations
- High-efficiency peaks with excellent recovery
- Higher capacity than other columns based on nonporous particles
- Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing
- Compatible with mass spectrometry (MS)
We recommend our CX-1 pH gradient buffers when using these columns with LC-UV/LC-FLD.
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文献和实验使用新型ACQUITY UPC2 Torus色谱柱分析药物降解产物
。但是，另一项技术——超高效合相色谱（UltraPerformance Convergence Chromatography™，UPC2®）的出现，使分析人员能够完成这类困难的分离工作。 解决方案 ACQUITY UPC2系统利用液态CO2作为流动相的优势，可对复杂样品（如，药物降解产物）进行分离。与反相色谱相比，UPC2技术可实现正交分离。借助UPC2平台提供的最新色谱柱技术——ACQUITY UPC2 Torus色谱柱，分析人员可对这类样品进行快速分析。本实验使用配备有新型ACQUTIY
Invitrogen 性能卓越的系统整合了可以利用的最好的RT技术，包括SuperScript™ III RT和最新推出的Thermo-X™ RT。SuperScript™ III RT是M-MLV RT基因工程升级版本，减少了RNaseH活性，提供更高的热稳定性（1-3）。这种酶可以在高达55℃的情况下合成cDNA，比以前使用的反转录酶提供更高的特异性，更高的cDNA产量以及更多的全长产物。Thermo-X™ RT克隆自一种嗜热真细菌，提供高达70℃的热稳定
基因治疗--BIA Separations！让病毒纯化更高效
）的抛光步骤，进一步从超螺旋（SC）治疗级 pDNA 中除去开环（OC）和线性 pDNA 亚型。 两步纯化的作用功能明确、互相合作，大大节省操作步骤和时间。 AEX 色谱柱（CIMmultus™DEAE）浓缩 pDNA，并去除大部分 HCP、mRNA 和基因组 DNA。 在弱阴离子交换柱上捕获质粒 DNA，其中除去剩余的 RNA 和蛋白质，样品结合需要低电导率，通过稀释实现。 随着增加 NaCl 的梯度洗脱，首先洗
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